Peculiarities of gene expression of the EcoRII modification-restriction system

被引:0
作者
Matvienko, NN
Zheleznaya, LA
Chernyshova, EE
Buryanov, YI
Matvienko, NI [1 ]
机构
[1] Russian Acad Sci, Inst Prot Res, Pushchino 142292, Moscow Region, Russia
[2] Russian Acad Sci, Branch Shemyakin Ovchinnikov Inst Bioorgan Chem, Pushchino 142292, Moscow Region, Russia
[3] Russian Acad Sci, Inst Theoret & Expt Biophys, Pushchino 142292, Moscow Region, Russia
关键词
restriction-modification; EcoRII;
D O I
暂无
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The restriction-modification genes of the EcoRII system have been cloned into plasmids under control of phage-specific promoters T7 and SP6. The transcription was induced by cell infection with the recombinant M13 phages with the corresponding genes of phage RNA-polymerases under control of the p(lac)-promoter in the presence of IPTG. The induction yields significant amounts of EcoRII DNA-methylase for both phage-specific promoters. In both cases no increase in EcoRII endonuclease expression could be achieved. We hypothesize that the expression of the endonuclease gene is regulated on the translational level.
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页码:1124 / 1127
页数:4
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