Chemical composition, antibacterial activity of Cyperus rotundus rhizomes essential oil against Staphylococcus aureus via membrane disruption and apoptosis pathway

被引:81
作者
Zhang, Liang-Liang [1 ,2 ]
Zhang, Li-Fang [2 ]
Hu, Qing-Ping [3 ]
Hao, Dong-Lin [1 ]
Xu, Jian-Guo [1 ]
机构
[1] Shanxi Normal Univ, Sch Food Sci, Linfen 041004, Peoples R China
[2] Shanxi Normal Univ, Sch Chem & Mat Sci, Linfen 041004, Peoples R China
[3] Shanxi Normal Univ, Sch Life Sci, Linfen 041004, Peoples R China
关键词
Cyperus rotundus; Essential oil; Antibacterial activity; Membrane disruption; Apoptosis pathway; Staphylococcus aureus; BACTERIAL-CELL DEATH; ESCHERICHIA-COLI; NUCLEIC-ACID; PINE NEEDLES; MECHANISM; ANTIOXIDANT; L; CINNAMALDEHYDE; EXTRACTS; TUBERS;
D O I
10.1016/j.foodcont.2017.05.016
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
The chemical composition, antibacterial activity and mechanism of essential oil from Cyperus rotundus rhizomes against Staphylococcus aureus were investigated in this study. Results showed that alpha-cyperone, cyperene and alpha-selinene were the major components of the essential oil. The essential oil exhibited strong antibacterial activity against S. aureus with the minimum inhibitory concentration (MIC) and minimum bactericide concentration (MBC) were 10 and 20 mg/rnL respectively, and the antibacterial effects increased with increasing essential oil concentrations and treatment time. The electric conductivity, cell membrane integrity, NPN uptake, and membrane potential assays demonstrated that essential oil disrupted the membrane integrity of S. aureus. Electron microscope observations further confirmed that essential oil destroyed cell membrane. Moreover, we found that essential oil could induce cells death of S. aureus through apoptosis pathway based on apoptosis analysis. These findings suggested that essential oil mainly exerted antibacterial activity by damaging cell membrane and membrane-mediated apoptosis pathway. (C) 2017 Elsevier Ltd. All rights reserved.
引用
收藏
页码:290 / 296
页数:7
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