Effective bioreduction of hexavalent chromium-contaminated water in fixed-film bioreactors

被引:10
作者
Williams, Peter J. [1 ]
Botes, Elsabe [1 ]
Maleke, Maleke M. [1 ]
Ojo, Abidemi [1 ]
DeFlaun, Mary F. [2 ]
Howell, Jim [2 ]
Borch, Robert [2 ]
Jordan, Robert [1 ]
van Heerden, Esta [1 ]
机构
[1] Univ Free State, Dept Microbial Biochem & Food Biotechnol, TIA Metagen Platform, Bloemfontein, South Africa
[2] Geosyntec Consultants, Ewing, NJ USA
基金
新加坡国家研究基金会;
关键词
Bioreduction; fixed-film reactor; hexavalent chromium; microbial diversity; ENTEROBACTER-CLOACAE SLD1A-1; CHROMATE REDUCTASE-ACTIVITY; POLYMERASE-CHAIN-REACTION; RIBOSOMAL-RNA; SP NOV; BACTERIUM; NITRATE; STRAIN; SELENATE; IMMOBILIZATION;
D O I
10.4314/wsa.v40i3.19
中图分类号
TV21 [水资源调查与水利规划];
学科分类号
081501 ;
摘要
Hexavalent chromium (Cr6+) contamination from a dolomite stone mine in Limpopo Province, South Africa, has resulted in extensive groundwater contamination. In order to circumvent any further negative environmental impact at this site, an effective and sustainable treatment strategy for the removal of up to 6.49 mg/l Cr6+ from the groundwater was developed. Laboratory-scale, continuous up-flow bioreactors were constructed to evaluate reduction of Cr6+, with a residence time of 24 h, an efficiency porosity of 44% and a flow rate of 1.5 ml/min. Stoichiometrically balancing terminal electron acceptors in the feed water with a selected electron donor, directed reactor balance for complete Cr6+ reduction. The microbial community shifted in relative dominance during operation to establish an optimal metal-reducing community, including Enterobacter cloacae, Flavobacterium sp. and Ralstonia sp., which achieved 100% reduction. Evaluation after reactor termination with SEM-EDX and XRD confirmed the establishment of biofilm on the reactor matrix, as well as trivalent chromium (Cr3+) precipitation within the reactor. Due to gravitational force, high concentrations of Cr3+ were found in the bottom third of the reactor. Based on the results from the laboratory investigation, a 24 000 l fixed-film pilot bioreactor was designed and constructed at this site. Influent flow rates, electron donor injection and automated sampling were remotely controlled by a programmable logic controller (PLC). Similar to the laboratory column study, steady state conditions could be achieved and successful Cr6+ reduction was evident. This is the first up-scaled, effective demonstration of a biological chromium(VI) bioremediation system in South Africa.
引用
收藏
页码:549 / 554
页数:6
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