Multi-stage continuous high cell density culture systems: A review

被引:35
作者
Chang, Ho Nam [1 ]
Jung, Kwonsu [1 ]
Choi, Jin-dal-rae [1 ]
Lee, Joon Chul [2 ]
Woo, Hee-Chul [3 ]
机构
[1] Korea Adv Inst Sci & Technol, Dept Chem & Biomol Engn, Taejon 305701, South Korea
[2] KITECH, Ansan 426910, Gyeonggi Do, South Korea
[3] Pukyong Natl Univ, Dept Chem Engn, Pusan 608739, South Korea
关键词
Multi-stage continuous bioreactors; High cell density culture; Product titer improvement; Productivity improvement; FED-BATCH CULTURE; HOLLOW-FIBER BIOREACTOR; RECOMBINANT ANTIBODY-PRODUCTION; FILTER PERFUSION SYSTEM; ETHANOL-PRODUCTION; ALCALIGENES-EUTROPHUS; MEMBRANE BIOREACTORS; RECYCLE BIOREACTOR; IMMOBILIZED CELLS; ESCHERICHIA-COLI;
D O I
10.1016/j.biotechadv.2014.01.004
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
A multi-stage continuous high cell density culture (MSC-HCDC) system makes it possible to achieve high productivity together with high product titer of many bioproducts. For long-term continuous operation of MSC-HCDC systems, the cell retention time and hydraulic retention time must be decoupled and strains (bacteria, yeast, plant, and animal cells) must be stable. MSC-HCDC systems are suitable for low-value high-volume extracellular products such as fuel ethanol, lactic acid or volatile fatty acids, and high-value products such as monoclonal antibodies as well as intracellular products such as polyhydroxybutyric acid (PHB), microbial lipids or a number of therapeutics. Better understanding of the fermentation kinetics of a specific product and reliable high-density culture methods for the product-generating microorganisms will facilitate timely industrialization of MSC-HCDC systems for products that are currently obtained in fed-batch bioreactors. (C) 2014 Published by Elsevier Inc.
引用
收藏
页码:514 / 525
页数:12
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