NIST mixed stain study 3: DNA quantitation accuracy and its influence on short tandem repeat multiplex signal intensity

被引：34

作者：

Kline, MC ^{[1
]}

Duewer, DL ^{[1
]}

Redman, JW ^{[1
]}

Butler, JM ^{[1
]}

机构：

[1] Natl Inst Stand & Technol, Chem Sci & Technol Lab, Gaithersburg, MD 20899 USA

来源：

ANALYTICAL CHEMISTRY | 2003年 / 75卷 / 10期

关键词：

D O I：

10.1021/ac026410i

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The Mixed Stain Study 3 (MSS3) interlaboratory challenge exercise evaluated the 2001 performance of STR multiplex DNA typing systems using a set of seven DNA extracts of designed concentration and composition. This initial report focuses on the linkages connecting the measurement of the concentration of DNA ([DNA]) to the observed STR multiplex signal intensities. There is a causal relationship between [DNA] measurement accuracy and the efficiency of STR multiplex analysis. There are no intrinsic measurement performance differences among the [DNA] measurement technologies reported. However, there are large differences in the efficiencies of amplification, separation, and detection among participants using the same nominal measurement systems.

引用

页码：2463 / 2469

页数：7

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