Capture of an early fusion-active conformation of HIV-1 gp41

被引：478

作者：

Furuta, RA

Wild, CT

Weng, YK

Weiss, CD

机构：

[1] US FDA, Off Vaccines, Ctr Biol Evaluat & Res, Bethesda, MD 20892 USA

[2] Biotech Res Labs, Gaithersburg, MD 20877 USA

来源：

NATURE STRUCTURAL BIOLOGY | 1998年 / 5卷 / 04期

关键词：

D O I：

10.1038/nsb0498-276

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Using an inhibitory synthetic peptide (DP-178) from HIV-1 gp31, we have trapped HIV-1 envelope glycoprotein (Env) undergoing conformational changes during virus entry. Our data show that DP-178 binds gp41 and inhibits Env-mediated membrane fusion after gp 120 interacts with cellular receptors, indicating that conformational changes involving the coiled coil domain of gp41 are required for entry. Capture of this fusion-active conformation of Env provides insights into the early events leading to Env-mediated membrane fusion.

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页码：276 / 279

页数：4

相关论文

共 28 条

[1] EPITOPE MAPPING AND TOPOLOGY OF BACULOVIRUS-EXPRESSED HIV-1 GP160 DETERMINED WITH A PANEL OF MURINE MONOCLONAL-ANTIBODIES [J].

ABACIOGLU, YH ;

FOUTS, TR ;

LAMAN, JD ;

CLAASSEN, E ;

PINCUS, SH ;

MOORE, JP ;

ROBY, CA ;

KAMINLEWIS, R ;

LEWIS, GK .

AIDS RESEARCH AND HUMAN RETROVIRUSES, 1994, 10 (04) :371-381

[2]

Berger EA, 1997, AIDS, V11, pS3

[3] A SPRING-LOADED MECHANISM FOR THE CONFORMATIONAL CHANGE OF INFLUENZA HEMAGGLUTININ [J].

CARR, CM ;

KIM, PS .

CELL, 1993, 73 (04) :823-832

[4] HEPTAD REPEAT SEQUENCES ARE LOCATED ADJACENT TO HYDROPHOBIC REGIONS IN SEVERAL TYPES OF VIRUS FUSION GLYCOPROTEINS [J].

CHAMBERS, P ;

PRINGLE, CR ;

EASTON, AJ .

JOURNAL OF GENERAL VIROLOGY, 1990, 71 :3075-3080

[5] Core structure of gp41 from the HIV envelope glycoprotein [J].

Chan, DC ;

Fass, D ;

Berger, JM ;

Kim, PS .

CELL, 1997, 89 (02) :263-273

[6] A MOLECULAR CLASP IN THE HUMAN-IMMUNODEFICIENCY-VIRUS (HIV) TYPE-1 TM PROTEIN DETERMINES THE ANTI-HIV ACTIVITY OF GP41 DERIVATIVES - IMPLICATION FOR VIRAL FUSION [J].

CHEN, CH ;

MATTHEWS, TJ ;

MCDANAL, CB ;

BOLOGNESI, DP ;

GREENBERG, ML .

JOURNAL OF VIROLOGY, 1995, 69 (06) :3771-3777

[7] Identification of a major co-receptor for primary isolates of HIV-1 [J].

Deng, HK ;

Liu, R ;

Ellmeier, W ;

Choe, S ;

Unutmaz, D ;

Burkhart, M ;

DiMarzio, P ;

Marmon, S ;

Sutton, RE ;

Hill, CM ;

Davis, CB ;

Peiper, SC ;

Schall, TJ ;

Littman, DR ;

Landau, NR .

NATURE, 1996, 381 (6584) :661-666

[8] How do viruses enter cells? The HIV coreceptors teach us a lesson of complexity [J].

Dimitrov, DS .

CELL, 1997, 91 (06) :721-730

[9] Virus-cell and cell-cell fusion [J].

Hernandez, LD ;

Hoffman, LR ;

Wolfsberg, TG ;

White, JM .

ANNUAL REVIEW OF CELL AND DEVELOPMENTAL BIOLOGY, 1996, 12 :627-661

[10] Inhibition of HIV type 1 infectivity by constrained alpha-helical peptides: Implications for the viral fusion mechanism [J].

Judice, JK ;

Tom, JYK ;

Huang, W ;

Wrin, T ;

Vennari, J ;

Petropoulos, CJ ;

McDowell, RS .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (25) :13426-13430