2-(Dibutylamino)ethyl acrylate as a highly efficient co-reactant of Ru(bpy)32+ electrochemiluminescence for selective detection of cysteine

被引：17

作者：

Yuan, Fan ^{[1
,3
]}

Hao, Kai ^{[2
,3
]}

Sheng, Shu ^{[2
,4
]}

Fereja, Tadesse Haile ^{[1
,4
]}

Ma, Xiangui ^{[1
,3
]}

Liu, Feng ^{[2
,4
]}

Zafar, Muhammad Nadeem ^{[1
,5
]}

Lou, Baohua ^{[1
,3
]}

Tian, Huayu ^{[2
,3
]}

Xu, Guobao ^{[1
,3
]}

机构：

[1] Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Electroanalyt Chem, Changchun 130022, Jilin, Peoples R China

[2] Chinese Acad Sci, Changchun Inst Appl Chem, Key Lab Polymer Ecomat, Changchun 130022, Jilin, Peoples R China

[3] Univ Sci & Technol China, Hefei 230026, Anhui, Peoples R China

[4] Chinese Acad Sci, Univ Chinese Acad Sci, Beijing 100049, Peoples R China

[5] Univ Gujrat, Dept Chem, Gujrat 50700, Pakistan

来源：

ELECTROCHIMICA ACTA | 2020年 / 329卷

基金：

中国国家自然科学基金;

关键词：

Electrochemiluminescence; 2-(Dibutylamino)ethyl acrylate; Cysteine; Co-reactant; Olefin group; ELECTROGENERATED CHEMILUMINESCENCE; LIGHT-EMISSION; SYSTEM; TRIS(2,2'-BIPYRIDYL)RUTHENIUM(II); NANOPARTICLES; HOMOCYSTEINE; PLATINUM; PROTEIN;

D O I：

10.1016/j.electacta.2019.135117

中图分类号：

O646 [电化学、电解、磁化学];

学科分类号：

081704 ;

摘要：

A highly efficient co-reactant of Ru(bpy)(3)(2+) electrochemiluminescence (ECL), 2-(dibutylamino)ethyl acrylate(DBAEA), was successfully synthesized by a straightforward one-step reaction between dibutylaminoethanol and acrylyl chloride. On account of the interaction between the olefin group of DBAEA and Pt electrode, the ECL intensity utilizing DBAEA as the co-reactant was increased dramatically, nearly as 10 times and 17 times as conventional co-reactants dibutyaminoethanol and tripropylamine. Since cysteine can remove the acrylate group of DBAEA specifically through conjugated addition/cyclization reaction, the ECL intensity of Ru(bpy)(3)(2+)/DBAEA was declined selectively. Therefore, cysteine was detected through this novel ECL system with high selectivity towards other amino acids. The linear range was between 10 mu M and 200 mu M with a limit of detection of 1.15 mu M. The cysteine in Dulbecco's Modified Eagle Medium (DMEM) was detected and satisfying recovery was obtained. (C) 2019 Elsevier Ltd. All rights reserved.

引用

页数：7

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