A Facile Method To Encapsulate Proteins in Silica Nanoparticles: Encapsulated Green Fluorescent Protein as a Robust Fluorescence Probe

被引：63

作者：

Cao, Aoneng ^{[1
]}

Ye, Zhangmei ^{[1
]}

Cai, Zhengwei ^{[1
]}

Dong, Erya ^{[1
]}

Yang, Xiaowei ^{[1
]}

Liu, Gangbo ^{[1
]}

Deng, Xiaoyong ^{[1
]}

Wang, Yanli ^{[1
]}

Yang, Sheng-Tao ^{[2
]}

Wang, Haifang ^{[1
]}

Wu, Minghong ^{[1
]}

Liu, Yuanfang ^{[1
,2
]}

机构：

[1] Shanghai Univ, Inst Nanochem & Nanobiol, Shanghai 200444, Peoples R China

[2] Peking Univ, Coll Chem & Mol Engn, Beijing Natl Lab Mol Sci, Beijing 100871, Peoples R China

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2010年 / 49卷 / 17期

关键词：

fluorescent probes; immobilization; nanoparticles; polyhistidine tag; proteins; QUANTUM DOTS; IN-VIVO; PROTEOMICS;

D O I：

10.1002/anie.200906883

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

(Figure Presented) Si'l vous plait? A facile and general method has been developed to encapsulate polyhistidine-tagged proteins in silica nanoparticles (NPs; gray, see picture) using calcium ions (yellow). The enhanced green fluorescence protein (EGFP) encapsulated in the silica NPs shows a substantial increase in fluorescence intensity and stability against denaturants, protease, and heat. © 2010 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

页码：3022 / 3025

页数：4

共 24 条

[1] Bioinspired enzyme encapsulation for biocatalysis [J].

Betancor, Lorena ;

Luckarift, Heather R. .

TRENDS IN BIOTECHNOLOGY, 2008, 26 (10) :566-572

[2] High throughput protein production for functional proteomics [J].

Braun, P ;

LaBaer, J .

TRENDS IN BIOTECHNOLOGY, 2003, 21 (09) :383-388

[3] Fluorescent proteins as a toolkit for in vivo imaging [J].

Chudakov, DM ;

Lukyanov, S ;

Lukyanov, KA .

TRENDS IN BIOTECHNOLOGY, 2005, 23 (12) :605-613

[4]

Derfus AM, 2004, NANO LETT, V4, P11, DOI 10.1021/nl0347334

[5] In vivo imaging of quantum dots encapsulated in phospholipid micelles [J].

Dubertret, B ;

Skourides, P ;

Norris, DJ ;

Noireaux, V ;

Brivanlou, AH ;

Libchaber, A .

SCIENCE, 2002, 298 (5599) :1759-1762

[6] ENCAPSULATION OF PROTEINS IN TRANSPARENT POROUS SILICATE-GLASSES PREPARED BY THE SOL-GEL METHOD [J].

ELLERBY, LM ;

NISHIDA, CR ;

NISHIDA, F ;

YAMANAKA, SA ;

DUNN, B ;

VALENTINE, JS ;

ZINK, JI .

SCIENCE, 1992, 255 (5048) :1113-1115

[7] Encapsulation and controlled release of biomolecules from silica microparticles [J].

Finnie, K. S. ;

Jacques, D. A. ;

McGann, M. J. ;

Blackford, M. G. ;

Barbe, C. J. .

JOURNAL OF MATERIALS CHEMISTRY, 2006, 16 (46) :4494-4498

[8] Long-term Persistence and Spectral Blue Shifting of Quantum Dots in Vivo [J].

Fitzpatrick, James A. J. ;

Andreko, Susan K. ;

Ernst, Lauren A. ;

Waggoner, Alan S. ;

Ballou, Byron ;

Bruchez, Marcel P. .

NANO LETTERS, 2009, 9 (07) :2736-2741

[9] Preparation of luminescent Cy5 doped core-shell SFNPs and its application as a near-infrared fluorescent marker [J].

He, Xiaoxiao ;

Chen, Jiyun ;

Wang, Kemin ;

Qin, Dilan ;

Tan, Weihong .

TALANTA, 2007, 72 (04) :1519-1526

[10] Nanobiocatalysis and its potential applications [J].

Kim, Jungbae ;

Grate, Jay W. ;

Wang, Ping .

TRENDS IN BIOTECHNOLOGY, 2008, 26 (11) :639-646

← 1 2 3 →