Detection of a homologue of bcl-2 in plant cells

被引：16

作者：

Dion, M

Chamberland, H

St-Michel, C

Plante, M

Darveau, A

Lafontaine, JG

Brisson, LF

机构：

[1] Univ Laval, Dept Biochem, Quebec City, PQ G1K 7P4, Canada

[2] Univ Laval, Dept Biol, Quebec City, PQ G1K 7P4, Canada

来源：

BIOCHEMISTRY AND CELL BIOLOGY-BIOCHIMIE ET BIOLOGIE CELLULAIRE | 1997年 / 75卷 / 04期

关键词：

bcl-2; apoptosis; plant cell; evolution; lymphocyte;

D O I：

10.1139/bcb-75-4-457

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An emerging family of bcl-2-like genes has been identified from nematode to humans. These genes play a role in the maintenance of homeostasis. Its members have highly conserved domains that are important for their dimerization. Since nothing is known about the importance of these genes in plant cells, we have investigated their presence in an alga as well as in three higher plants both by Western analysis and by immunocytochemistry. Immunoblots revealed the presence of a protein immunoreacting with the anti-bcl-2 polyclonal antibody in leaves of tobacco plants. Furthermore, immunocytochemical localization has shown that this protein is mainly associated with mitochondria, plastids, and nuclei of plant cells. Taken together, our results suggest that bcl-2 is a protein highly conserved throughout evolution.

引用

页码：457 / 461

页数：5

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