The effect of isoflurane on the release of [3H]-acetylcholine from rat brain cortical slices

Volatile general anaesthetics are believed to affect synaptic transmission, but their actions in the central nervous system (CNS) remains unclear. Acetylcholine (ACh) is one of the most important neurotransmitter in the CNS and thus, it is possible that its release could be one of the targets for volatile anaesthetic action. However, the effects of these agents on the release of ACh are not yet fully understood. Rat brain cortical slices were loaded with [H-3]-choline in order to study the effect of isoflurane on the release of [H-3]-ACh from this preparation. Isoflurane (28, 43, 54, 95 and 182 nM) significantly increased the basal release of [H-3]-ACh. This effect was independent of the extracellular sodium and calcium concentration but was decreased by tetracaine and dantrolene, inhibitors of Ca(2+-)release from intracellular stores. These findings indicate that isoflurane may cause a Ca(2+-)release from internal stores that increases [H-3]-ACh release in rat brain cortical slices. (C) 2000 Elsevier Science Inc.