Circular dichroism spectroscopy: An efficient approach for the quantitation of ampicillin in presence of cloxacillin

被引：17

作者：

Rahman, Nafisur ^{[1
]}

Khan, Sumaiya ^{[1
]}

机构：

[1] Aligarh Muslim Univ, Dept Chem, Aligarh 202002, Uttar Pradesh, India

来源：

SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY | 2016年 / 160卷

关键词：

Circular dichroism spectra; Ampicillin; Cloxacillin; Validation; Pharmaceutical formulation; BETA-LACTAM ANTIBIOTICS; CAPILLARY-ELECTROPHORESIS; PENICILLIN-G; HUMAN URINE; VALIDATION; EXTRACTION; PHARMACEUTICALS; CEPHALOSPORINS; CHROMATOGRAPHY; LABORATORIES;

D O I：

10.1016/j.saa.2016.02.009

中图分类号：

O433 [光谱学];

学科分类号：

0703 ; 070302 ;

摘要：

Ampicillin exhibited a negative and a positive cotton effects on the circular dichroism (CD) spectra in the wavelength range of 200-280 nm. Cloxacillin showed a positive cotton band peaking at 228 nm. Three sensitive, precise and accurate CD spectroscopic methods have been developed for the determination of ampicillin and cloxacillin. Method A was used for the determination of ampicillin in presence of cloxacillin by measuring ellipticity at 206 nm. Method B and C were employed to determine ampicillin and cloxacillin based on evaluation of ellipticity at 233 nm and 228 nm, respectively. Methods A, B and C showed linearity in the concentration range of 10-40 mu g mL(-1), 5-40 mu g mL(-1) ampicillin and 10-80 mu g mL(-1) cloxacillin, respectively. The method A was successfully applied to the determination of ampicillin in commercial dosage forms containing equivalent amount of cloxacillin. (C) 2016 Elsevier B.V. All rights reserved.

引用

页码：26 / 33

页数：8

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