Evaluation of Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for Identification of Vibrio cholerae

被引:7
|
作者
Rychert, Jenna [1 ,2 ,3 ]
Creely, David [5 ,6 ,7 ]
Mayo-Smith, Leslie M. [3 ]
Calderwood, Stephen B. [2 ,3 ]
Ivers, Louise C. [8 ,9 ]
Ryan, Edward T. [2 ,3 ]
Boncy, Jacques [10 ]
Qadri, Firdausi [11 ]
Ahmed, Dilruba [11 ]
Ferraro, Mary Jane [1 ,2 ]
Harris, Jason B. [2 ,3 ,4 ]
机构
[1] Massachusetts Gen Hosp, Dept Pathol, Boston, MA 02114 USA
[2] Massachusetts Gen Hosp, Dept Med, Boston, MA 02114 USA
[3] Massachusetts Gen Hosp, Div Infect Dis, Boston, MA 02114 USA
[4] Massachusetts Gen Hosp, Dept Pediat, Boston, MA 02114 USA
[5] Massachusetts Gen Hosp, Boston, MA 02114 USA
[6] Harvard Univ, Sch Med, Boston, MA USA
[7] bioMerieux, Hazelwood, MO USA
[8] Harvard Univ, Sch Med, Dept Global Hlth & Social Med, Boston, MA USA
[9] Partners Hlth, Boston, MA USA
[10] Natl Publ Hlth Lab, Port Au Prince, Haiti
[11] Int Ctr Diarrhoeal Dis Res, Dhaka 1000, Bangladesh
关键词
FAMILY VIBRIONACEAE; MEMBERS;
D O I
10.1128/JCM.02666-14
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
We evaluated the use of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MS) for the identification of Vibrio cholerae. MS identified all 42 isolates of V. cholerae O1 and O139 and 7 of 9 non-O1/O139 isolates. MS correctly discriminated between all Aeromonas and V. cholerae isolates. Overall, MS performed as well as or better than biochemical methods.
引用
收藏
页码:329 / 331
页数:3
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