A Genome-Wide Scan for Signatures of Selection in Kurdish Horse Breed

The genetic diversity and genomic regions being under putative natural selection in Kurdish horse population were studied. The samples from 72 horses were genotyped by using GGP Equine 70K SNP arrays. The Ne Slope (NeS) analyses revealed that a sharp decline in Ne has probably occurred around four generations ago, and high frequency of ROH with 2-4 Mbp in length suggested that the inbreeding has probably occurred around 20 generations ago. The effective population size (Ne) was 104 horses up to three generations ago and the average inbreeding (F-ROH) was 0.047( +/- 0.045). Using de-correlated composite of multiple selection signals (DCMS) and runs of homozygosity (ROH) analyses the genomic regions being under putative selection were detected. By using DCMS, a total of 148 significant SNP (FDR < 0.05) were identified, 40% of which were located on ECA9, where the greatest peak was observed. This genomic region harbors several known QTL which are associated with withers height (body size). Also, signifi-cant genomic regions (FDR < 0.05), harboring QTL associated with insect bite hypersensitivity (IBH), hair density and coat texture, alternate gaits, guttural pouch tympany and temperament were identified. By using outputs of ROH analyses, two hotspot regions (i.e., 30% of individuals was considered as threshold), were identified on ECA7 (50.11-54.36 Mbp) and ECA11 (26.10-29.07 Mbp) harboring QTL associated with withers height, alternate gait and IBH. In summary, the genomic regions being under putative natural selection which harbors known QTL associated with body size and IBH, among others, were introduced. Nevertheless, additional functional and comparative studies are necessary to corroborate their effect on the observed genetic and phenotypic diversity of the Kurdish horses. (c) 2022 Elsevier Inc. All rights reserved.