. Yeast RNA-Binding Protein Nab3 Regulates Genes Involved in Nitrogen Metabolism

被引：10

作者：

Merran, Jonathan ^{[1
]}

Corden, Jeffry L. ^{[1
]}

机构：

[1] Johns Hopkins Univ, Sch Med, Dept Mol Biol & Genet, Baltimore, MD 21218 USA

来源：

MOLECULAR AND CELLULAR BIOLOGY | 2017年 / 37卷 / 18期

关键词：

nitrogen metabolism; noncoding RNA; termination; transcription; POLYMERASE-II TRANSCRIPTION; CRYPTIC UNSTABLE TRANSCRIPTS; GATA FACTOR RESPONSES; BIDIRECTIONAL PROMOTERS; RAPAMYCIN TREATMENT; ACTIVATORS GLN3; TERMINATION; PHOSPHORYLATION; EXPRESSION; NRD1;

D O I：

10.1128/MCB.00154-17

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Termination of Saccharomyces cerevisiae RNA polymerase II (Pol II) transcripts occurs through two alternative pathways. Termination of mRNAs is coupled to cleavage and polyadenylation while noncoding transcripts are terminated through the Nrd1-Nab3-Sen1 (NNS) pathway in a process that is linked to RNA degradation by the nuclear exosome. Some mRNA transcripts are also attenuated through premature termination directed by the NNS complex. In this paper we present the results of nuclear depletion of the NNS component Nab3. As expected, many noncoding RNAs fail to terminate properly. In addition, we observe that nitrogen catabolite-repressed genes are upregulated by Nab3 depletion.

引用

页数：11

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