C-reactive protein specifically enhances platelet-activating factor-induced inflammatory activity in vivo

被引:7
作者
Sato, Akira [1 ]
Oe, Keitaro [1 ]
Yamanaka, Hikaru [1 ]
Yokoyama, Izumi [1 ]
Ebina, Keiichi [1 ]
机构
[1] Iwaki Meisei Univ, Fac Pharm, Iwaki, Fukushima 9708551, Japan
关键词
C-reactive protein; Platelet-activating factor; Inflammation; Oedema; Inflammatory disease; FACTOR PAF RECEPTOR; OXIDIZED-LDL; ENDOTHELIAL-CELLS; BINDING; LYSOPHOSPHATIDYLCHOLINE; RAT; 1-O-HEXADECYL-2-ACETYL-SN-GLYCERO-3-PHOSPHOCHOLINE; IDENTIFICATION; EXPRESSION; LIGAND;
D O I
10.1016/j.ejphar.2014.10.020
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
Platelet-activating factor (PAP) is a potent lipid mediator that is implicated in numerous inflammatory diseases. C-reactive protein (CRP) is an acute-phase plasma protein that increases rapidly and dramatically in response to inflammation. In this study, we investigated the effect of the interaction between CRP and PAF on inflammatory responses in vivo. From binding analysis using a time-resolved fluorometric assay, CRP bound to PAF and its precursor/metabolite lyso-PAF in a concentration-dependent manner. In addition, CRP bound to several phospholipids containing lysophosphatidylcholine, which bears structural resemblance to PAF and lyso-PAF, sphingosylphosphorylcholine, and lysophosphaticlylethanolamine more readily than to lysophosphatidic acid and lysophosphatidylserine. In in vivo experiments using a rat model of hind paw oedema, CRP increased PAF-induced rat paw oedema in a dose-dependent manner, without causing the oedema itself, but it did not increase histamine and serotonin-induced paw oedema. Furthermore, the receptor for CRP, lectin-like oxidized low-density lipoprotein receptor 1 was not involved in the increase in PAP-induced inflammatory responses caused by CRP. These results indicate that CRP can specifically enhance PAP-induced inflammatory activity through binding to PAP and lyso-PAR Therefore, CRP may accelerate the pathogenesis of numerous inflammatory diseases caused by PAR (C) 2014 Elsevier B.V. All rights reserved.
引用
收藏
页码:46 / 51
页数:6
相关论文
共 51 条
[21]  
Huang YH, 1999, CLIN EXP IMMUNOL, V116, P326
[22]   Agonist-induced sequestration, recycling, and resensitization of platelet-activating factor receptor - Role of cytoplasmic tail phosphorylation in each process [J].
Ishii, I ;
Saito, E ;
Izumi, T ;
Ui, M ;
Shimizu, T .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (16) :9878-9885
[23]   Platelet-activating factor (PAF) receptor and genetically engineered PAF receptor mutant mice [J].
Ishii, S ;
Shimizu, T .
PROGRESS IN LIPID RESEARCH, 2000, 39 (01) :41-82
[24]   Evidence for the contribution of tumour necrosis factor in oedema formation induced by histamine in the hind paw of the rat [J].
Kalokasidis, K. ;
Molyva, D. ;
Mirtsou, V. ;
Kokkas, B. ;
Goulas, A. .
INFLAMMATION RESEARCH, 2009, 58 (08) :437-440
[25]  
Karthikeyan Ramaraju, 2014, Perspect Clin Res, V5, P75, DOI 10.4103/2229-3485.128026
[26]   Platelet-activating factor production in the spinal cord of experimental allergic encephalomyelitis mice via the group IVA cytosolic phospholipase A2-Lyso-PAFAT axis [J].
Kihara, Yasuyuki ;
Yanagida, Keisuke ;
Masago, Kayo ;
Kita, Yoshihiro ;
Hishikawa, Daisuke ;
Shindou, Hideo ;
Ishii, Satoshi ;
Shimizu, Takao .
JOURNAL OF IMMUNOLOGY, 2008, 181 (07) :5008-5014
[27]  
Kita T, 2000, ANN NY ACAD SCI, V902, P95
[28]   AUGMENTATION OF 1-0-ALKYL-2-0-ACETYL-SN-GLYCERO-3-PHOSPHOCHOLINE (PAF)-INDUCED HUMAN-PLATELET ACTIVATION BY C-REACTIVE PROTEIN [J].
KOHAYAKAWA, M ;
INOUE, K .
THROMBOSIS RESEARCH, 1986, 41 (05) :649-657
[29]  
Marathe GK, 2001, J LIPID RES, V42, P1430
[30]   CULTURED ENDOTHELIAL-CELLS SYNTHESIZE BOTH PLATELET-ACTIVATING FACTOR AND PROSTACYCLIN IN RESPONSE TO HISTAMINE, BRADYKININ, AND ADENOSINE-TRIPHOSPHATE [J].
MCINTYRE, TM ;
ZIMMERMAN, GA ;
SATOH, K ;
PRESCOTT, SM .
JOURNAL OF CLINICAL INVESTIGATION, 1985, 76 (01) :271-280