Techniques and strategies employing engineered transcription factors

被引：2

作者：

Lawson, Seth ^{[1
,2
]}

Bleris, Leonidas ^{[1
,2
,3
]}

机构：

[1] Univ Texas Dallas, Bioengn Dept, Richardson, TX 75083 USA

[2] Univ Texas Dallas, Ctr Syst Biol, Richardson, TX 75083 USA

[3] Univ Texas Dallas, Dept Biol Sci, Richardson, TX USA

来源：

CURRENT OPINION IN BIOMEDICAL ENGINEERING | 2017年 / 4卷

基金：

美国国家科学基金会;

关键词：

DNA-binding domains; dCas9; Genome editing; Transcriptional activators; Screens; Spatiotemporal control;

D O I：

10.1016/j.cobme.2017.10.009

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Programmable transcription factors have been instrumental in recent elucidations of developmental and regulatory pathways pertaining to biology and medicine. Programmable DNA-binding domains (DBDs) are cheap and relatively easy to use. Moreover, fusing transcriptional and epigenetic modulators to programmable DBDs can be done with ease. Recent improvements of these modulators have increased the efficacy and appeal of their biomedical applications. Screening and spatiotemporal control of genomic regulation using programmable DBDs have drastically improved the breadth and resolution of (epi)genomic knowledge. The widespread adoption of programmable DBDs for (epi)genomic investigations will lead to a deeper and more comprehensive understanding of diseases and other biological phenomena.

引用

页码：152 / 162

页数：11

共 118 条

[1] Genomic Copy Number Dictates a Gene-Independent Cell Response to CRISPR/Cas9 Targeting [J].

Aguirre, Andrew J. ;

Meyers, Robin M. ;

Weir, Barbara A. ;

Vazquez, Francisca ;

Zhang, Cheng-Zhong ;

Ben-David, Uri ;

Cook, April ;

Ha, Gavin ;

Harrington, William F. ;

Doshi, Mihir B. ;

Kost-Alimova, Maria ;

Gill, Stanley ;

Xu, Han ;

Ali, Levi D. ;

Jiang, Guozhi ;

Pantel, Sasha ;

Lee, Yenarae ;

Goodale, Amy ;

Cherniack, Andrew D. ;

Oh, Coyin ;

Kryukov, Gregory ;

Cowley, Glenn S. ;

Garraway, Levi A. ;

Stegmaier, Kimberly ;

Roberts, Charles W. ;

Golub, Todd R. ;

Meyerson, Matthew ;

Root, David E. ;

Tsherniak, Aviad ;

Hahn, William C. .

CANCER DISCOVERY, 2016, 6 (08) :914-929

[2] Conditionally Stabilized dCas9 Activator for Controlling Gene Expression in Human Cell Reprogramming and Differentiation [J].

Balboa, Diego ;

Weltner, Jere ;

Eurola, Solja ;

Trokovic, Ras ;

Wartiovaara, Kirmo ;

Otonkoski, Timo .

STEM CELL REPORTS, 2015, 5 (03) :448-459

[3] An engineered tale-transcription factor rescues transcription of factor VII impaired by promoter mutations and enhances its endogenous expression in hepatocytes [J].

Barbon, Elena ;

Pignani, Silvia ;

Branchini, Alessio ;

Bernardi, Francesco ;

Pinotti, Mirko ;

Bovolenta, Matteo .

SCIENTIFIC REPORTS, 2016, 6

[4] CRISPR provides acquired resistance against viruses in prokaryotes [J].

Barrangou, Rodolphe ;

Fremaux, Christophe ;

Deveau, Helene ;

Richards, Melissa ;

Boyaval, Patrick ;

Moineau, Sylvain ;

Romero, Dennis A. ;

Horvath, Philippe .

SCIENCE, 2007, 315 (5819) :1709-1712

[5] 3′ UTR seed matches, but not overall identity, are associated with RNAi off-targets [J].

Birmingham, A ;

Anderson, EM ;

Reynolds, A ;

Ilsley-Tyree, D ;

Leake, D ;

Fedorov, Y ;

Baskerville, S ;

Maksimova, E ;

Robinson, K ;

Karpilow, J ;

Marshall, WS ;

Khvorova, A .

NATURE METHODS, 2006, 3 (03) :199-204

[6] Breaking the Code of DNA Binding Specificity of TAL-Type III Effectors [J].

Boch, Jens ;

Scholze, Heidi ;

Schornack, Sebastian ;

Landgraf, Angelika ;

Hahn, Simone ;

Kay, Sabine ;

Lahaye, Thomas ;

Nickstadt, Anja ;

Bonas, Ulla .

SCIENCE, 2009, 326 (5959) :1509-1512

[7] Versatile in vivo regulation of tumor phenotypes by dCas9-mediated transcriptional perturbation [J].

Braun, Christian J. ;

Bruno, Peter M. ;

Horlbeck, Max A. ;

Gilbert, Luke A. ;

Weissman, Jonathan S. ;

Hemann, Michael T. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2016, 113 (27) :E3892-E3900

[8] Efficient design and assembly of custom TALEN and other TAL effector-based constructs for DNA targeting [J].

Cermak, Tomas ;

Doyle, Erin L. ;

Christian, Michelle ;

Wang, Li ;

Zhang, Yong ;

Schmidt, Clarice ;

Baller, Joshua A. ;

Somia, Nikunj V. ;

Bogdanove, Adam J. ;

Voytas, Daniel F. .

NUCLEIC ACIDS RESEARCH, 2011, 39 (12) :e82

[9]

Chavez A, 2016, NAT METHODS, V13, P563, DOI [10.1038/NMETH.3871, 10.1038/nmeth.3871]

[10]

Chavez A, 2015, NAT METHODS, V12, P326, DOI [10.1038/nmeth.3312, 10.1038/NMETH.3312]

← 1 2 3 4 5 6 7 8 9 10 →