Biotemplated Synthesis of Gold Nanoparticle-Bacteria Cellulose Nanofiber Nanocomposites and Their Application in Biosensing

被引：294

作者：

Zhang, Taiji ^{[1
]}

Wang, Wei ^{[1
]}

Zhang, Dayong ^{[1
]}

Zhang, Xinxiang ^{[1
]}

Ma, Yurong ^{[1
]}

Zhou, Yinglin ^{[1
]}

Qi, Limin ^{[1
]}

机构：

[1] Peking Univ, Key Lab Biochem & Mol Engn, BNLMS,Coll Chem, State Key Lab Struct Chem Unstable & Stable Speci, Beijing 100871, Peoples R China

来源：

ADVANCED FUNCTIONAL MATERIALS | 2010年 / 20卷 / 07期

关键词：

HORSERADISH-PEROXIDASE; METAL NANOPARTICLES; CARBON NANOTUBES; HYBRID MATERIAL; COLLOIDAL GOLD; NANOWIRES; ASSEMBLIES; VIRUS; ELECTROCHEMISTRY; IMMOBILIZATION;

D O I：

10.1002/adfm.200902104

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Bacteria cellulose (BC) nanofibers are used as robust biotemplates for the facile fabrication of novel gold nanoparticle (NP)-bacteria cellulose nanofiber (Au-BC) nanocomposites via a one-step method. The BC nanofibers are uniformly coated with Au NPs in aqueous suspension using poly(ethyleneimine) (PEI) as the reducing and linking agent. With the addition of different halides, Au-BC nanocomposites with different Au shell thicknesses are formed, and a possible formation mechanism is proposed by taking into account the special role played by PEI. A novel H2O2 biosensor is constructed using the obtained Au BC nanocomposites as excellent support for horseradish peroxidase (HRP) immobilization, which allows the detection of H2O2 with a detection limit lower than 1 mu M. The Au-BC nanocomposites could be further used for the immobilization of many other enzymes, and thus, may find potential applications in bioelectroanalysis and bioelectrocatalysis.

引用

页码：1152 / 1160

页数：9