Integrated microreaction system for optical resolution of racemic amino acids

被引：42
作者：
Honda, Takeshi
Miyazaki, Masaya
Yamaguchi, Yoshiko
Nakamura, Hiroyuki
Maeda, Hideaki
机构：
[1] AIST, Nanotechnol Res Inst, Saga, Japan
[2] Kyushu Univ, Interdisciplinary Grad Sch Engn Sci, Dept Mol & Mat Sci, Kasuga, Fukuoka 816, Japan
来源：
LAB ON A CHIP | 2007年 / 7卷 / 03期
关键词：
D O I：
10.1039/b614500k
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
We developed a novel microreaction system for optical resolution of racemic amino acids. This device, which is based on a continuous microfluidic system, consists of an enzyme-immobilized microreactor and a microextractor. Use of the enzyme-microreactor, which was prepared by membrane formation on the microchannel surface, enabled a highly enantioselective reaction for a racemic amino acid derivative. The microextractor provided a laminar flow of two immiscible solutions, which enabled selective extraction of the product. Using this integrated device, we could perform efficient continuous production of optically pure unnatural amino acids.
引用
收藏
页码：366 / 372
页数：7
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