Extraction of Genomic DNA via Superparamagnetic Fe3O4 Magnetic Colloidal Nanocrystal Clusters

被引：7
作者：
Zhang, Jie [1 ]
Zhu, Mingsong [1 ]
Yang, Yajun [1 ]
Cao, Junjun [1 ]
Shi, Feng [1 ]
机构：
[1] Shihezi Univ, Sch Life Sci, Xinjiang 832003, Peoples R China
来源：
JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY | 2018年 / 18卷 / 12期
基金：
中国国家自然科学基金;
关键词：
Fe3O4 Magnetic Colloidal Nanocrystal Clusters; Silica-Coated Fe3O4 Magnetic Colloidal Nanocrystal Clusters; Genomic DNA Extraction; Whole Blood; Escherichia coli; Staphylococcus aureus; RAPID DETECTION; NANOPARTICLES; CHEMILUMINESCENCE; SEPARATION;
D O I：
10.1166/jnn.2018.16399
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
We herein describe a solvothermal method for preparing superparamagnetic Fe3O4 magnetic colloidal nanocrystal clusters with an average diameter of 195 nm. The Fe3O4 magnetic colloidal nanocrystal clusters were characterized by transmission electron microscopy (TEM), dynamic light scattering (DLS) and other methods. The Fe3O4 magnetic colloidal nanocrystal clusters were used to extract genomic DNA from fresh blood, Gram-negative bacteria, Gram-positive bacteria and frozen blood. Results showed that the genomic DNA integrity, purity and yield extracted via this universal method were satisfactory. This method is rapid, simple and does not use protease K or toxic compounds. This method lays the foundation for future research and nucleic acid extraction techniques.
引用
收藏
页码：8105 / 8110
页数：6
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