Qualitative and event-specific PCR real-time detection methods for StarLink maize

被引:52
作者
Windels, P
Bertrand, S
Depicker, A
Moens, W
Bockstaele, E
Loose, M
机构
[1] Agr Res Ctr, Dept Genet & Plant Breeding, B-9090 Melle, Belgium
[2] Inst Publ Hlth, Sect Biosafety & Biotechnol, B-1050 Brussels, Belgium
[3] State Univ Ghent VIB, B-9000 Ghent, Belgium
[4] Univ Ghent, Dept Plant Prod, B-9000 Ghent, Belgium
关键词
StarLink maize; junction region; GMO; identification; PCR;
D O I
10.1007/s00217-002-0652-5
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
In this paper we present qualitative detection and identification methods for StarLink maize (event CBH-351). The methodology proposed envisages detection of an internal target site in the cry9c coding region, as well as two event-specific target sites at the junction between the CBH-351 insert DNA and the genomic plant DNA. The cry9c-specific primer pair, generating a 180 bp amplicon, has been tested and optimised for conventional end-point PCR amplification. The event-specific primer pairs, generating amplicons of 138 bp and 100 bp respectively, give good performance in a conventional end-point PCR and in a real-time PCR assay. Our results clearly demonstrate that the primer pairs proposed can be used in an unambiguous and specific PCR identification assay for StarLink maize.
引用
收藏
页码:259 / 263
页数:5
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