Differential Proteomics Identification of HSP90 as Potential Serum Biomarker in Hepatocellular Carcinoma by Two-dimensional Electrophoresis and Mass Spectrometry

被引：43

作者：

Sun, Yiyi ^{[2
]}

Zang, Zhihe ^{[2
]}

Xu, Xiaohong ^{[2
]}

Zhang, Zhonglin ^{[2
]}

Zhong, Ling ^{[2
]}

Zan, Wang ^{[2
]}

Zhao, Yan ^{[2
]}

Sun, Lin ^{[1
]}

机构：

[1] Sichuan Univ, W China Hosp, Chengdu 610041, Sichuan Prov, Peoples R China

[2] Chengdu Med Coll, Chengdu 610083, Sichuan Prov, Peoples R China

来源：

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2010年 / 11卷 / 04期

关键词：

proteomics; hepatocellular carcinoma; serum biomarker; IMMOBILIZED PH GRADIENTS; SHOCK-PROTEIN; 90; GEL-ELECTROPHORESIS; COLORECTAL-CANCER; GENE-EXPRESSION; EARLY-DIAGNOSIS; BREAST-CANCER; TUMOR-MARKERS; EPIDEMIOLOGY; INHIBITORS;

D O I：

10.3390/ijms11041423

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The aim of the current study is to identify the potential biomarkers involved in Hepatocellular carcinoma (HCC) carcinogenesis. A comparative proteomics approach was utilized to identify the differentially expressed proteins in the serum of 10 HCC patients and 10 controls. A total of 12 significantly altered proteins were identified by mass spectrometry. Of the 12 proteins identified, HSP90 was one of the most significantly altered proteins and its over-expression in the serum of 20 HCC patients was confirmed using ELISA analysis. The observations suggest that HSP90 might be a potential biomarker for early diagnosis, prognosis, and monitoring in the therapy of HCC. This work demonstrates that a comprehensive strategy of proteomic identification combined with further validation should be adopted in the field of cancer biomarker discovery.

引用

页码：1423 / 1433

页数：11

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