Identification of a novel anticancer mechanism of Paeoniae Radix extracts based on systematic transcriptome analysis

被引：15

作者：

Baek, Su-Jin ^{[1
]}

Lee, Haeseung ^{[1
,2
]}

Park, Sang-Min ^{[1
]}

Park, Musun ^{[1
]}

Yi, Jin-Mu ^{[3
]}

Kim, No Soo ^{[3
]}

Kim, Aeyung ^{[4
]}

Cha, Seongwon ^{[1
]}

机构：

[1] Korea Inst Oriental Med, Korean Med KM Data Div, Daejeon 34054, South Korea

[2] Pusan Natl Univ, Coll Pharm, Busan 46241, South Korea

[3] Korea Inst Oriental Med, Korean Med KM Convergence Res Div, Daejeon 34054, South Korea

[4] Korea Inst Oriental Med, Korean Med KM Applicat Ctr, Daegu 41062, South Korea

来源：

BIOMEDICINE & PHARMACOTHERAPY | 2022年 / 148卷

关键词：

Lung cancer; Paeoniae Radix; Aurora B pathway; Transcriptome; LUNG-CANCER GROWTH; AURORA-B; KINASE INHIBITORS; CONNECTIVITY MAP; CELLS; PROLIFERATION; APOPTOSIS; TARGET;

D O I：

10.1016/j.biopha.2022.112748

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Paeoniae Radix (PR) has a great therapeutic value in many clinical applications; however, the presence of various bioactive compounds and its complicated effects on human health makes its precise mechanisms of action unclear. This study investigated the effects of PR at the molecular pathway level by profiling genome-wide gene expression changes following dose-dependent treatment of human lung cancer cells (A549) with PR water extract (WPR), PR ethanol extracts (EPR), as well as their individual components. We found that PR exerts anticancer effects in A549 cells by regulating numerous pathways. Specifically, EPR and two compounds, namely, hederagenin (HG) and oleanolic acid (OA), significantly downregulate the Aurora B pathway. Furthermore, we generated an integrated PR extracts-compounds-target genes network in the Aurora B pathway to understand their interactions. Our findings reinforce that inhibiting Aurora kinase activity is a therapeutic target for treating cancers, providing the potential for novel mechanisms of action for PR and its components against lung cancer.

引用

页数：11

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