Preparation and characterization of Cu,Zn-superoxide dismutase covalently modified by polyunsaturated fatty acids

被引：7

作者：

Zhan, Z. J. ^{[1
]}

Zhou, Z. G. ^{[1
]}

Shan, W. G. ^{[1
]}

机构：

[1] Zhejiang Univ Technol, Coll Pharmaceut Sci, Hangzhou 310014, Zhejiang, Peoples R China

来源：

BIOCHEMISTRY-MOSCOW | 2009年 / 74卷 / 11期

关键词：

Cu; Zn-superoxide dismutase; polyunsaturated fatty acids; chemical modification; stability; partition coefficient; ALPHA-LINOLENIC ACID; SUPEROXIDE-DISMUTASE; CANCER-CELLS; HYPERTROPHY; INHIBITION; PATHWAYS; CATALASE; PLASMA; TISSUE; LIPIDS;

D O I：

10.1134/S0006297909110133

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

To improve its stability and lipophilicity, Cu,Zn-superoxide dismutase (SOD) was chemically modified with linoleic and alpha-linolenic acids using two different methods. Higher retained enzymatic activity has been observed compared with SOD modified by macromolecular substances. Enhanced heat stability, acid and alkali resistance, and anti-pepsin/trypsin ability of the modified SOD were observed compared with those of the natural enzyme, the apparent oil-water partition coefficient being especially increased. The results characterize SOD modified with polyunsaturated fatty acids as a promising pharmacological tool.

引用

页码：1266 / 1269

页数：4

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