Preparation and characterization of Cu,Zn-superoxide dismutase covalently modified by polyunsaturated fatty acids

被引：7

作者：

Zhan, Z. J. ^{[1
]}

Zhou, Z. G. ^{[1
]}

Shan, W. G. ^{[1
]}

机构：

[1] Zhejiang Univ Technol, Coll Pharmaceut Sci, Hangzhou 310014, Zhejiang, Peoples R China

来源：

BIOCHEMISTRY-MOSCOW | 2009年 / 74卷 / 11期

关键词：

Cu; Zn-superoxide dismutase; polyunsaturated fatty acids; chemical modification; stability; partition coefficient; ALPHA-LINOLENIC ACID; SUPEROXIDE-DISMUTASE; CANCER-CELLS; HYPERTROPHY; INHIBITION; PATHWAYS; CATALASE; PLASMA; TISSUE; LIPIDS;

D O I：

10.1134/S0006297909110133

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

To improve its stability and lipophilicity, Cu,Zn-superoxide dismutase (SOD) was chemically modified with linoleic and alpha-linolenic acids using two different methods. Higher retained enzymatic activity has been observed compared with SOD modified by macromolecular substances. Enhanced heat stability, acid and alkali resistance, and anti-pepsin/trypsin ability of the modified SOD were observed compared with those of the natural enzyme, the apparent oil-water partition coefficient being especially increased. The results characterize SOD modified with polyunsaturated fatty acids as a promising pharmacological tool.

引用

页码：1266 / 1269

页数：4

共 50 条

[31] CU, ZN-SUPEROXIDE DISMUTASE (SOD) LOCALIZATION IN THE GLOMERULI OF IGA NEPHROPATHY [J].

FUTENMA, A ;

YAMADA, H ;

MIYAI, H ;

YAMADA, K ;

IIDA, T ;

KATO, K .

JOURNAL OF CLINICAL BIOCHEMISTRY AND NUTRITION, 1991, 11 (01) :59-67

[32] Dry powder inhaler formulation of Cu,Zn-superoxide dismutase by spray drying: A proof-of-concept [J].

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[33] Good and Bad of Cu/Zn-Superoxide Dismutase Controlled by Metal Ions and Disulfide Bonds [J].

Furukawa, Yoshiaki .

CHEMISTRY LETTERS, 2021, 50 (02) :331-341

[34] Cu,Zn-superoxide dismutase gene dosage and cell resistance to oxidative stress: A review [J].

Peskin, AV .

BIOSCIENCE REPORTS, 1997, 17 (01) :85-89

[35] INDUCTION OF CU,ZN-SUPEROXIDE DISMUTASE AFTER CORTICAL CONTUSION INJURY DURING HYPOTHERMIA [J].

FUKUHARA, T ;

NISHIO, S ;

ONO, Y ;

KAWAUCHI, M ;

ASARI, S ;

OHMOTO, T .

BRAIN RESEARCH, 1994, 657 (1-2) :333-336

[36] A Primary Role for Disulfide Formation in the Productive Folding of Prokaryotic Cu,Zn-superoxide Dismutase [J].

Sakurai, Yasuyuki ;

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[37] REGULATION OF CU,ZN-SUPEROXIDE AND MN-SUPEROXIDE DISMUTASE TRANSCRIPTION IN SACCHAROMYCES-CEREVISIAE [J].

GALIAZZO, F ;

LABBEBOIS, R .

FEBS LETTERS, 1993, 315 (02) :197-200

[38] Steered molecular dynamic simulations of conformational lock of Cu, Zn-superoxide dismutase [J].

Xiao, Bao-Lin ;

Ning, Yan-Na ;

Niu, Nan-Nan ;

Li, Di ;

Moosavi-Movahedi, Ali Akbar ;

Sheibani, Nader ;

Hong, Jun .

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[39] EXCESS COPPER INDUCES A CYTOSOLIC CU, ZN-SUPEROXIDE DISMUTASE IN SOYBEAN ROOT [J].

CHONGPRADITNUN, P ;

MORI, S ;

CHINO, M .

PLANT AND CELL PHYSIOLOGY, 1992, 33 (03) :239-244

[40] Aggregation of α-synuclein induced by the Cu,Zn-superoxide dismutase and hydrogen peroxide system [J].

Kim, KS ;

Choi, SY ;

Kwon, HY ;

Woo, MH ;

Kang, TC ;

Kang, JH .

FREE RADICAL BIOLOGY AND MEDICINE, 2002, 32 (06) :544-550

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