Preparation and characterization of Cu,Zn-superoxide dismutase covalently modified by polyunsaturated fatty acids

被引：7
作者：
Zhan, Z. J. [1 ]
Zhou, Z. G. [1 ]
Shan, W. G. [1 ]
机构：
[1] Zhejiang Univ Technol, Coll Pharmaceut Sci, Hangzhou 310014, Zhejiang, Peoples R China
来源：
BIOCHEMISTRY-MOSCOW | 2009年 / 74卷 / 11期
关键词：
Cu; Zn-superoxide dismutase; polyunsaturated fatty acids; chemical modification; stability; partition coefficient; ALPHA-LINOLENIC ACID; SUPEROXIDE-DISMUTASE; CANCER-CELLS; HYPERTROPHY; INHIBITION; PATHWAYS; CATALASE; PLASMA; TISSUE; LIPIDS;
D O I：
10.1134/S0006297909110133
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
To improve its stability and lipophilicity, Cu,Zn-superoxide dismutase (SOD) was chemically modified with linoleic and alpha-linolenic acids using two different methods. Higher retained enzymatic activity has been observed compared with SOD modified by macromolecular substances. Enhanced heat stability, acid and alkali resistance, and anti-pepsin/trypsin ability of the modified SOD were observed compared with those of the natural enzyme, the apparent oil-water partition coefficient being especially increased. The results characterize SOD modified with polyunsaturated fatty acids as a promising pharmacological tool.
引用
收藏
页码：1266 / 1269
页数：4
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