Selective cleavage of protonated penetratin and its substitutes under low-energy collision-induced dissociation condition

被引：7

作者：

Peng, Juan ^{[1
]}

Zu, Lily ^{[1
]}

Fang, Weihai ^{[1
]}

Huang, Lingyun ^{[2
]}

Wang, Yaru ^{[1
]}

He, Dacheng ^{[2
]}

机构：

[1] Beijing Normal Univ, Dept Chem, Beijing 100875, Peoples R China

[2] Beijing Normal Univ, Key Lab Cell Proliferat & Regulat, Minist Educ, Beijing 100875, Peoples R China

来源：

JOURNAL OF MASS SPECTROMETRY | 2010年 / 45卷 / 06期

基金：

中国国家自然科学基金;

关键词：

penetratin; substitutes; selective cleavage; collision-induced dissociation; ESI-Q-TOF MS/MS; MAIN FRAGMENTATION PATHWAYS; GAS-PHASE FRAGMENTATION; TANDEM MASS-SPECTRA; GLN-GLY CLEAVAGE; AB-INITIO; ION CHEMISTRY; AMINO-ACIDS; AMIDE BOND; PEPTIDES; OLIGOPEPTIDES;

D O I：

10.1002/jms.1748

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An understanding of the dissociation of penetratin is important for improving its metabolic stability and cargo-delivery efficiency. In this study, we describe the selective cleavage of the K15-K16 amide bond of penetratin under the low-energy collision-induced dissociation condition in mass spectrometry. A variety of penetratin substitutes have been studied in which key basic amino acids have been substituted within the sequence. The calculated structure indicates that an a-helix structure prevents the fragmentation of the central peptide domain and the side chain of lysine is involved in the proton translocation process. Copyright (C) 2010 John Wiley & Sons, Ltd.

引用

页码：627 / 634

页数：8

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