An Electrochemical Biosensor for Detection of DNA Species Related to Oral Cancer Based on a Particular Host-Guest Recognition-Assisted Strategy for Signal Tag In Situ

被引:8
作者
Hu, Yuanru [1 ]
Chang, Yuanyuan [1 ]
Chai, Yaqin [1 ]
Yuan, Ruo [1 ]
机构
[1] Southwest Univ, Coll Chem & Chem Engn, Minist Educ, Key Lab Luminescence & Real Time Analyt Chem, Chongqing 400715, Peoples R China
基金
中国国家自然科学基金;
关键词
GRAPHENE; IMMOBILIZATION; AMPLIFICATION; NANOPARTICLE; OXIDE; IMMUNOSENSOR; FABRICATION; PLATFORM; GROWTH; SENSOR;
D O I
10.1149/2.0851807jes
中图分类号
O646 [电化学、电解、磁化学];
学科分类号
081704 ;
摘要
Herein, a novel host-guest recognition among tryptophan (Trp)-linkedDNA, methyl viologen (MV2+) and cucurbit[ 8] uril (CB[8]) was introduced to construct a biosensor for the sensitive assay of oral cancer gene based on nicking enzyme signaling amplification (NESA) and Pt & Pd-MoS2 nanomaterial for the first time. A bioconjugates containing alkaline phosphatase (ALP) and complementary strand (S1) was designed as signal probe to catalyze the 1-naphthyl phosphate monosodium salt monohydrate (NPP) to in situ generate massive electroactive substance 1-naphthol, resulting in the generation of significant peak current with use of Pt & Pd-MoS2 nanomaterial as a catalyst. With target recycling amplification and highly efficient catalysis of ALP, the proposed biosensor for target DNA exhibited a satisfactory linear range from 10 fM to 10 nM. Importantly, the admirable performance of the proposed method shows a potential in biomedical research and clinical diagnosis. (c) 2018 The Electrochemical Society.
引用
收藏
页码:B289 / B295
页数:7
相关论文
共 48 条
[1]   Impedimetic immunosensor for the label-free and direct detection of botulinum neurotoxin serotype A using Au nanoparticles/graphene-chitosan composite [J].
Afkhami, Abbas ;
Hashemi, Pegah ;
Bagheri, Hasan ;
Salimian, Jafar ;
Ahmadi, Ali ;
Madrakian, Tayyebeh .
BIOSENSORS & BIOELECTRONICS, 2017, 93 :124-131
[2]   Protein capped Cu nanoclusters-SWCNT nanocomposite as a novel candidate of high performance platform for organophosphates enzymeless biosensor [J].
Bagheri, Hasan ;
Afkhami, Abbas ;
Khoshsafar, Hosein ;
Hajian, Ali ;
Shahriyari, Alireza .
BIOSENSORS & BIOELECTRONICS, 2017, 89 :829-836
[3]   Modified fractal iron oxide magnetic nanostructure: A novel and high performance platform for redox protein immobilization, direct electrochemistry and bioelectrocatalysis application [J].
Bagheri, Hasan ;
Ranjbari, Elias ;
Amiri-Aref, Mohaddeseh ;
Hajian, Ali ;
Ardakani, Yalda Hosseinzadeh ;
Amidi, Salimeh .
BIOSENSORS & BIOELECTRONICS, 2016, 85 :814-821
[4]   ELP-OPH/BSA/TiO2 nanofibers/c-MWCNTs based biosensor for sensitive and selective determination of p-nitrophenyl substituted organophosphate pesticides in aqueous system [J].
Bao, Jing ;
Hou, Changjun ;
Dong, Qiuchen ;
Ma, Xiaoyu ;
Chen, Jun ;
Huo, Danqun ;
Yang, Mei ;
Abd El Galil, Khaled Hussein ;
Chen, Wilfred ;
Lei, Yu .
BIOSENSORS & BIOELECTRONICS, 2016, 85 :935-942
[5]   Noncovalent Chirality Sensing Ensembles for the Detection and Reaction Monitoring of Amino Acids, Peptides, Proteins, and Aromatic Drugs [J].
Biedermann, Frank ;
Nau, Werner M. .
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2014, 53 (22) :5694-5699
[6]   Charge-mediated recognition of N-terminal tryptophan in aqueous solution by a synthetic host [J].
Bush, ME ;
Bouley, ND ;
Urbach, AR .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2005, 127 (41) :14511-14517
[7]   An ultrasensitive electrochemical biosensor for detection of DNA species related to oral cancer based on nuclease-assisted target recycling and amplification of DNAzyme [J].
Chen, JingHua ;
Zhang, Jing ;
Guo, Yang ;
Li, Juan ;
Fu, FengFu ;
Yang, Huang-Hao ;
Chen, Guonan .
CHEMICAL COMMUNICATIONS, 2011, 47 (28) :8004-8006
[8]   An electrochemical DNA biosensor based on nitrogen-doped graphene/Au nanoparticles for human multidrug resistance gene detection [J].
Chen, Mei ;
Hou, Changjun ;
Huo, Danqun ;
Bao, Jing ;
Fa, Huanbao ;
Shen, Caihong .
BIOSENSORS & BIOELECTRONICS, 2016, 85 :684-691
[9]   Molecular Recognition of Insulin by a Synthetic Receptor [J].
Chinai, Jordan M. ;
Taylor, Alexander B. ;
Ryno, Lisa M. ;
Hargreaves, Nicholas D. ;
Morris, Christopher A. ;
Hart, P. John ;
Urbach, Adam R. .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2011, 133 (23) :8810-8813
[10]   CD 59 Targeted Ultrasensitive Electrochemical Immunosensor for Fast and Noninvasive Diagnosis of Oral Cancer [J].
Choudhary, Meenakshi ;
Yadav, Prashant ;
Singh, Anu ;
Kaur, Satbir ;
Ramirez-Vick, Jaime ;
Chandra, Pranjal ;
Arora, Kavita ;
Singh, Surinder P. .
ELECTROANALYSIS, 2016, 28 (10) :2565-2574