Mutation of key residues in the C-methyltransferase domain of a fungal highly reducing polyketide synthase

被引：11

作者：

Skellam, Elizabeth J. ^{[1
]}

Hurley, Deirdre ^{[1
]}

Davison, Jack ^{[1
]}

Lazarus, Colin M. ^{[2
]}

Simpson, Thomas J. ^{[1
]}

Cox, Russell J. ^{[1
]}

机构：

[1] Univ Bristol, Sch Chem, Bristol BS8 1TS, Avon, England

[2] Univ Bristol, Sch Biol Sci, Bristol BS8 1TS, Avon, England

来源：

MOLECULAR BIOSYSTEMS | 2010年 / 6卷 / 04期

基金：

英国工程与自然科学研究理事会; 英国生物技术与生命科学研究理事会;

关键词：

FATTY-ACID SYNTHASE; FUSARIUM-VERTICILLIOIDES; HETEROLOGOUS EXPRESSION; SQUALENE SYNTHASE; CRYSTAL-STRUCTURE; BETA-OXIDATION; BIOSYNTHESIS; GENE; PROTEINS; CLONING;

D O I：

10.1039/b923990a

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Site directed mutations of the C-methyltransferase domain of squalestatin tetraketide synthase were made in an attempt to alter the methylation pattern of the synthase expressed in vivo: mutation resulted in either no effect or in complete abrogation of polyketide production.

引用

页码：680 / 682

页数：3

共 22 条

[1] Improvement of compactin (ML-236B) production by genetic engineering in compactin high-producing Penicillium citrinum
Baba, S.
Abe, Y.
Suzuki, T.
Ono, C.
Iwamoto, K.
Nihira, T.
Hosobuchi, M.
[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2009, 83 (04) : 697 - 704
[2] Squalestatin cures prion-infected neurons and protects against prion neurotoxicity
Bate, C
Salmona, M
Diomede, L
Williams, A
[J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2004, 279 (15) : 14983 - 14990
[3] BAXTER A, 1992, J BIOL CHEM, V267, P11705
[4] Conformational flexibility of metazoan fatty acid synthase enables catalysis
Brignole, Edward J.
Smith, Stuart
Asturias, Francisco J.
[J]. NATURE STRUCTURAL & MOLECULAR BIOLOGY, 2009, 16 (02) : 190 - 197
[5] Rapid cloning and expression of a fungal polyketide synthase gene involved in squalestatin biosynthesis
Cox, RJ
Glod, F
Hurley, D
Lazarus, CM
Nicholson, TP
Rudd, BAM
Simpson, TJ
Wilkinson, B
Zhang, Y
[J]. CHEMICAL COMMUNICATIONS, 2004, (20) : 2260 - 2261
[6] Polyketides, proteins and genes in fungi: programmed nano-machines begin to reveal their secrets
Cox, Russell J.
[J]. ORGANIC & BIOMOLECULAR CHEMISTRY, 2007, 5 (13) : 2010 - 2026
[7] Crystal structure of glycine N-methyltransferase from rat liver
Fu, ZJ
Hu, YB
Konishi, K
Takata, Y
Ogawa, H
Gomi, T
Fujioka, M
Takusagawa, F
[J]. BIOCHEMISTRY, 1996, 35 (37) : 11985 - 11993
[8] Cloning of the polyketide synthase gene atX from Aspergillus terreus and its identification as the 6-methylsalicylic acid synthase gene by heterologous expression
Fujii, I
Ono, Y
Tada, H
Gomi, K
Ebizuka, Y
Sankawa, U
[J]. MOLECULAR AND GENERAL GENETICS, 1996, 253 (1-2): : 1 - 10
[9] Authentic heterologous expression of the tenellin iterative polyketide synthase nonribosomal peptide synthetase requires coexpression with an enoyl reductase
Halo, Laura M.
Marshall, James W.
Yakasai, Ahmed A.
Song, Zhongshu
Butts, Craig P.
Crump, Matthew P.
Heneghan, Mary
Bailey, Andrew M.
Simpson, Thomas J.
Lazarus, Colin. M.
Cox, Russell J.
[J]. CHEMBIOCHEM, 2008, 9 (04) : 585 - 594
[10] HILTUNEN JK, 1992, J BIOL CHEM, V267, P6646

← 1 2 3 →