PREVALENCE AND SOME VIRULENCE GENES OF ESCHERICHIA COLI O157 ISOLATED FROM CHICKEN MEATS AND GIBLETS

被引：4

作者：

Guran, Husnu Sahan ^{[1
]}

Vural, Aydin ^{[1
]}

Erkan, Mehmet Emin ^{[1
]}

Durmusoglu, Halil ^{[1
]}

机构：

[1] Dicle Univ, Dept Food Hyg & Technol, Fac Vet Med, TR-21280 Diyarbakir, Turkey

来源：

ANNALS OF ANIMAL SCIENCE | 2017年 / 17卷 / 02期

关键词：

E. coli O157; chicken meat; stx(1); stx(2); eaeA; hlyA; MOLECULAR CHARACTERIZATION; BOVINE FECES; PRODUCTS; POULTRY; PCR; GASTROENTERITIS; IDENTIFICATION; BURGERS; NUMBERS; BROILER;

D O I：

10.1515/aoas-2016-0061

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

Escherichia coli O157 related foodborne illnesses continue to be one of the most important global public health problems in the world. This study aims to determine E. coli O157 prevalence in 375 chicken meat parts and giblets. The samples were collected randomly from several supermarkets and butchers in Diyarbakir, a city in southeast Turkey. They were analyzed and confirmed using the immunomagnetic separation (IMS), Vitek (R) 2 microbial identification system and polymerase chain reaction (PCR) method. This study also aims to detect the presence of fliC(H7), eaeA, stx(1), stx(2) and hlyA genes by using PCR. The overall E. coli O157 prevalence in chicken meat parts and giblets was 1.3%. All of the E. coli O157 isolates carried rfbE(O157) and eaeA genes; but not any fliC(H7) and hlyA genes. The E. coli O157 isolates obtained from drumstick and breast meat carried either stx(1) or stx(2) genes, which were related to important virulence factors of the disease.

引用

页码：555 / 563

页数：9

共 36 条

[1] Prevalence and potential link between E. coli O157:H7 isolated from drinking water, meat and vegetables and stools of diarrhoeic confirmed and non-confirmed HIV/AIDS patients in the Amathole District -: South Africa [J].