Hyperbaric oxygen preconditioning ameliorates hypoxia-ischemia brain damage by activating Nrf2 expression in vivo and in vitro

被引：22
作者：
Zhai, Xiao [1 ,2 ]
Lin, Han [3 ]
Chen, Yu [3 ]
Chen, Xiao [2 ]
Shi, Jiazi [1 ]
Chen, Ouyang [1 ]
Li, Jiasi [4 ]
Sun, Xuejun [5 ]
机构：
[1] Second Mil Med Univ, Changhai Hosp, Grad Management Unit, Shanghai, Peoples R China
[2] Second Mil Med Univ, Changhai Hosp, Dept Orthoped, Shanghai, Peoples R China
[3] Second Mil Med Univ, Changhai Hosp, Dept Gastroenterol, Shanghai, Peoples R China
[4] Second Mil Med Univ, Changhai Hosp, Dept Neurol, Shanghai, Peoples R China
[5] Second Mil Med Univ, Fac Naval Med, Shanghai, Peoples R China
来源：
FREE RADICAL RESEARCH | 2016年 / 50卷 / 04期
关键词：
Hyperbaric oxygen; hypoxia-ischemia brain damage; ischemic tolerance; prevention; reactive oxygen species; TRANSFERASE GENE-EXPRESSION; NEONATAL-RAT MODEL; OXIDATIVE STRESS; HEME OXYGENASE-1; REPERFUSION INJURY; ENDOTHELIAL-CELLS; UP-REGULATION; CORTICAL-NEURONS; INDUCTION; TOLERANCE;
D O I：
10.3109/10715762.2015.1136411
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The present study aimed to investigate whether hyperbaric oxygen preconditioning (HBO-PC) could ameliorate hypoxia-ischemia brain damage (HIBD) by an increase of Nrf2 expression. P7 Sprague-Dawley rats (aged 7 d, n=195) were used in two in vivo experiments, including BO-PC exposure experiments in non-HIBD models and treatment experiments in HIBD models. 2,3,5-triphenyltetrazolium chloride (TTC) staining, Nissl Staining, and TUNEL staining were performed. And expressions of Nrf2, HO-1, and GSTs were measured. For in vitro studies, oxygen-glucose deprivation cells were established. Morphological and apoptotic staining and gene silencing of Nrf2 by siRNA transfection were investigated. For exposure experiments, HBO-PC for longer time increased the expression of Nrf2 significantly. And for treatment experiments, HBO-PC treatment significantly decreased infarction area, lessened neuronal injury, reduced apoptosis, and increased both the expression of Nrf2 and activities of its downstream proteins. Cytology tests confirmed effects of HBO-PC treatments. Besides, Nrf2 siRNA significantly reduced protective effects of HBO-PC. These observations demonstrated that an up-regulation of Nrf2 by HBO-PC might play an important role in the generation of tolerance against HIBD.
引用
收藏
页码：454 / 466
页数：13
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