Lumbar Myeloid Cell Trafficking into Locomotor Networks after Thoracic Spinal Cord Injury

被引:15
作者
Hansen, Christopher N. [1 ,2 ,3 ]
Norden, Diana M. [1 ,2 ,3 ]
Faw, Timothy D. [1 ,2 ,3 ,4 ]
Deibert, Rochelle [1 ,2 ]
Wohleb, Eric S. [3 ,5 ]
Sheridan, John F. [1 ,5 ,6 ]
Godbout, Jonathan P. [1 ,3 ,6 ]
Basso, D. Michele [1 ,2 ]
机构
[1] Ohio State Univ, Ctr Brain & Spinal Cord Repair, Columbus, OH 43210 USA
[2] Ohio State Univ, Sch Hlth & Rehabil Sci, 106 Atwell Hall,453 W 10th Ave, Columbus, OH 43210 USA
[3] Ohio State Univ, Dept Neurosci, Columbus, OH 43210 USA
[4] Ohio State Univ, Neurosci Grad Program, Columbus, OH 43210 USA
[5] Ohio State Univ, Div Biosci, Columbus, OH 43210 USA
[6] Ohio State Univ, Inst Behav Med Res, Columbus, OH 43210 USA
关键词
Spinal cord injury; Inflammation; MMP-9; Macrophage; Blood brain barrier; ANXIETY-LIKE BEHAVIOR; BLOOD-BRAIN-BARRIER; CONTUSION INJURY; NEUROPATHIC PAIN; FUNCTIONAL RECOVERY; ENDOTHELIAL-CELLS; VASCULAR EVENTS; MICROGLIA; ACTIVATION; MOUSE;
D O I
10.1016/j.expneurol.2016.05.019
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Spinal cord injury (SCI) promotes inflammation along the neuroaxis that jeopardizes plasticity, intrinsic repair and recovery. While inflammation at the injury site is well-established, less is known within remote spinal networks. The presence of bone marrow-derived immune (myeloid) cells in these areas may further impede functional recovery. Previously, high levels of the gelatinase, matrix metalloproteinase-9 (MMP-9) occurred within the lumbar enlargement after thoracic SCI and impeded activity-dependent recovery. Since SCI-induced MMP-9 potentially increases vascular permeability, myeloid cell infiltration may drive inflammatory toxicity in loco motor networks. Therefore, we examined neurovascular reactivity and myeloid cell infiltration in the lumbar cord after thoracic SCI. We show evidence of region-specific recruitment of myeloid cells into the lumbar but not cervical region. Myeloid infiltration occurred with concomitant increases in chemoattractants (CCL2) and cell adhesion molecules (ICAM-1) around lumbar vasculature 24 h and 7 days post injury. Bone marrow GFP chimeric mice established robust infiltration of bone marrow-derived myeloid cells into the lumbar gray matter 24 h after SCI. This cell infiltration occurred when the blood-spinal cord barrier was intact, suggesting active recruitment across the endothelium. Myeloid cells persisted as ramified macrophages at 7 days post injury in parallel with increased inhibitory GAD67 labeling. Importantly, macrophage infiltration required MMP-9. (C) 2016 Elsevier Inc. All rights reserved.
引用
收藏
页码:86 / 98
页数:13
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