Determination of proteins at nanogram levels based on their enhancement effects of Rayleigh light scattering on dibromomethylchlorophosphonazo

被引：16
|
作者：
Li, QF [1 ]
Zhang, HY [1 ]
Xue, CX [1 ]
Chen, XG [1 ]
Hu, ZD [1 ]
机构：
[1] Lanzhou Univ, Dept Chem, Lanzhou 730000, Peoples R China
来源：
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY | 2000年 / 56卷 / 12期
基金：
中国博士后科学基金;
关键词：
protein assay; Rayleigh light scattering spectroscopy; dibromomethylchlorophosphonazo;
D O I：
10.1016/S1386-1425(00)00329-2
中图分类号：
O433 [光谱学];
学科分类号：
0703 ; 070302 ;
摘要：
A new Rayleigh light scattering (RLS) assay of protein was conducted in this paper. At the optimum pH conditions, and in the presence of Tween-20, the weak RLS of dibromomethylchlorophosphonazo (DBM-CPA) can be enhanced greatly by the addition of proteins. Based on this, the reactions of DBM-CPA and proteins were studied. A new quantitative determination method for proteins has been developed. The method is simple, practical and relatively free from interference from coexisting substances, as well as much more sensitive (the dynamic ranges of 0.065-40.05 mu g ml(-1) and detection limit of 30 ng ml(-1) for bovine serum albumin (BSA)) than most of the existing assays. The determination results of human body serum samples are identical to those by the CBB method, with relative S.D, of six determination of 0.5-2.2%. (C) 2000 Elsevier Science B.V. All rights reserved.
引用
收藏
页码：2465 / 2470
页数：6
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