Plasma Extracellular Vesicle miRNAs Can Identify Lung Cancer, Current Smoking Status, and Stable COPD

被引:28
作者
O'Farrell, Hannah E. [1 ]
Bowman, Rayleen V. [1 ]
Fong, Kwun M. [1 ]
Yang, Ian A. [1 ]
机构
[1] Univ Queensland, Prince Charles Hosp, Thorac Res Ctr, Chermside, Qld 4032, Australia
关键词
extracellular vesicles; miRNAs; plasma; lung cancer; chronic obstructive pulmonary disease; OBSTRUCTIVE PULMONARY-DISEASE; NORMALIZATION STRATEGIES; DOWN-REGULATION; MICRORNAS; EXPRESSION; BIOMARKERS; METASTASIS; SYNDECAN-1; EXOSOMES; MARKER;
D O I
10.3390/ijms22115803
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Lung cancer remains the leading cause of cancer related mortality worldwide. We aimed to test whether a simple blood biomarker (extracellular vesicle miRNAs) can discriminate between cases with and without lung cancer. Methods: plasma extracellular vesicles (EVs) were isolated from four cohorts (n = 20 in each): healthy non-smokers, healthy smokers, lung cancer, and stable COPD participants. EV miRNA expression was evaluated using the miRCURY LNA miRNA Serum/Plasma assay for 179 specific targets. Significantly dysregulated miRNAs were assessed for discriminatory power using ROC curve analysis. Results: 15 miRNAs were differentially expressed between lung cancer and healthy non-smoking participants, with the greatest single miRNA being miR-205-5p (AUC 0.850), improving to AUC 0.993 in combination with miR-199a-5p. Moreover, 26 miRNAs were significantly dysregulated between lung cancer and healthy smoking participants, with the greatest single miRNA being miR-497-5p (AUC 0.873), improving to AUC 0.953 in combination with miR-22-5p; 14 miRNAs were significantly dysregulated between lung cancer and stable COPD participants, with the greatest single miRNA being miR-27a-3p (AUC 0.803), with two other miRNAs (miR-106b-3p and miR-361-5p) further improving discriminatory power (AUC 0.870). Conclusion: this case control study suggests miRNAs in EVs from plasma holds key biological information specific for lung cancer and warrants further prospective assessment.
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页数:19
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