Alpha-fetoprotein producing cells act as cancer progenitor cells in human cholangiocarcinoma

被引：31

作者：

Ishii, Takamichi ^{[1
,2
]}

Yasuchika, Kentaro ^{[2
]}

Suemori, Hirofumi

Nakatsuji, Norio ^{[3
,4
]}

Ikai, Iwao ^{[2
,5
]}

Uemoto, Shinji ^{[2
]}

机构：

[1] Kyoto Univ, Inst Frontier Med Sci, Stem Cell Res Ctr, Lab Embryon Stem Cell Res,Sakyo Ku, Kyoto 6068507, Japan

[2] Kyoto Univ, Grad Sch Med, Dept Surg, Kyoto 6068507, Japan

[3] Kyoto Univ, Inst Integrated Cell Mat Sci, Kyoto 6068507, Japan

[4] Kyoto Univ, Inst Frontier Med Sci, Dept Dev & Differentiat, Kyoto 6068507, Japan

[5] Natl Hosp Org, Kyoto Med Ctr, Dept Surg, Kyoto, Japan

来源：

CANCER LETTERS | 2010年 / 294卷 / 01期

关键词：

Cancer stem cell; Cholangiocarcinoma; AFP; Notch; ICC; EMBRYONIC STEM-CELLS; PROSPECTIVE IDENTIFICATION; LIVER-CANCER; DIFFERENTIATION; HEPATOCYTES; MATURATION; MARKERS; TARGET; MICE;

D O I：

10.1016/j.canlet.2010.01.019

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

We aimed to demonstrate that alpha-fetoprotein (AFP)-producing cells in cholangiocarcinomas possessed cancer stem cell (CSC)-like properties. AFP enhancer/promoter-driven EGFP gene was transfected into human cholangiocarcinoma cell lines. One cell line, RBE, expressed both AFP and EGFP. Clonal analyses revealed that one EGFP-positive cell generated both EGFP-positive and EGFP-negative cell fractions. However, one EGFP-negative cell never produced EGFP-positive cells. The EGFP-positive cells had a greater tumorigenic potential. Only the EGFP-positive cells expressed Notch1. AFP and Notch1 expression was observed in clinical intrahepatic cholangiocarcinomas. The AFP-producing cells were suggested to be CSCs. The Notch pathway might play an important role in maintaining the CSC characteristics. (C) 2010 Elsevier Ireland Ltd. All rights reserved.

引用

页码：25 / 34

页数：10

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