Identification of a tRNA-specific nuclear export receptor

被引：316
作者：
Kutay, U
Lipowsky, G
Izaurralde, E
Bischoff, FR
Schwarzmaier, P
Hartmann, E
Gorlich, D [1 ]
机构：
[1] Heidelberg Univ, Zentrum Mol Biol, D-69120 Heidelberg, Germany
[2] Univ Geneva, Dept Mol Biol, CH-1211 Geneva 4, Switzerland
[3] Deutsch Krebsforschungszentrum, Abt Mol Biol Mitose, D-69120 Heidelberg, Germany
[4] Max Delbruck Ctr Mol Med, D-13122 Berlin, Germany
来源：
MOLECULAR CELL | 1998年 / 1卷 / 03期
关键词：
D O I：
10.1016/S1097-2765(00)80036-2
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
In eukaryotes, tRNAs are synthesized in the nucleus and after several maturation steps exported to the cytoplasm. Here, we identify exportin-t as a specific mediator of tRNA export. It is a RanGTP-binding, importin beta-related factor with predominantly nuclear localization. It shuttles rapidly between nucleus and cytoplasm and interacts with nuclear pore complexes. Exportin-t binds tRNA directly and with high affinity. Its cellular concentration in Xenopus oocytes was found to be rate-limiting for export of all tRNAs tested, as judged by microinjection experiments. RanGTP regulates the substrate-exportin-t interaction such that tRNA can be preferentially bound in the nucleus and released in the cytoplasm.
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页码：359 / 369
页数：11
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