Fluorescence/Luminescence Circadian Imaging of Complex Tissues at Single-Cell Resolution

被引：14

作者：

Sellix, Michael T. ^{[1
]}

Currie, Jake ^{[1
]}

Menaker, Michael ^{[1
]}

Wijnen, Herman ^{[1
]}

机构：

[1] Univ Virginia, Dept Biol, Charlottesville, VA 22904 USA

来源：

JOURNAL OF BIOLOGICAL RHYTHMS | 2010年 / 25卷 / 03期

基金：

美国国家科学基金会;

关键词：

circadian clock; Drosophila; imaging; luciferase; luminescence; fluorescence; gene expression; GENE-EXPRESSION; CLOCK; TIME; PERIOD;

D O I：

10.1177/0748730410368016

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The use of luciferase reporter genes together with luminescence detection has enabled high frequency monitoring of molecular circadian clock function in living tissues. With the help of an intensified CCD camera combined with an inverted epifluorescence microscope, the authors have established a new imaging strategy that makes use of transgenic cell type-specific expression of fluorescent proteins to identify cells of interest for subsequent circadian luminescence recording at single-cell resolution.

引用

页码：228 / 232

页数：5

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