Transcriptomics-Based Study of Differentially Expressed Genes Related to Fat Deposition in Tibetan and Yorkshire Pigs

被引：27
作者：
Gong, Xinglong [1 ,2 ]
Zheng, Min [1 ,2 ]
Zhang, Jian [1 ,2 ]
Ye, Yourong [1 ,2 ]
Duan, Mengqi [1 ,2 ]
Chamba, Yangzom [1 ,2 ]
Wang, Zhongbin [1 ,2 ]
Shang, Peng [1 ,2 ]
机构：
[1] Tibet Agr & Anim Husb Coll, Tibet, Peoples R China
[2] Prov & Ministerial Cofounded Collaborat Innovat Ct, Tibet, Peoples R China
来源：
FRONTIERS IN VETERINARY SCIENCE | 2022年 / 9卷
基金：
中国国家自然科学基金;
关键词：
Tibetan pig; large white pig; fat deposition; transcriptomic; backfat;
D O I：
10.3389/fvets.2022.919904
中图分类号：
S85 [动物医学（兽医学）];
学科分类号：
0906 ;
摘要：
Fat deposition traits are one of the key factors in pig production and breeding. The fat deposition capacity of pigs mainly affects the quality of pork and pig productivity. The aim of this study was to analyze the differential expression of mRNA levels in dorsal adipose tissue of Tibetan and York pigs at different growth stages using transcriptomic data to estimate key genes that regulate fat deposition in pigs. The results showed that a total of 32,747 positively expressed genes were present in the dorsal adipose tissue of the two breeds. Differentially expressed gene (DEG) screening of multiple combinations between the two breeds yielded 324 DEGS. Gene ontology (GO) biofunctional enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses showed that these DEGS were mainly involved in lipid metabolic pathways, steroid biosynthetic pathways and lipid biosynthetic processes, sterol biosynthetic processes, brown adipocyte differentiation, and other pathways related to lipid deposition and metabolism. The results showed that ACACA, SLC2A4 and THRSP genes positively regulated the lipid deposition ability and CHPT1 gene negatively regulated the lipid deposition ability in pigs. The results of this experiment suggest a theoretical basis for further studies on the regulatory mechanisms of fat deposition in pigs.
引用
收藏
页数：9
相关论文
共 32 条
[1]   Elucidation of a promoter activity that directs the expression of acetyl-CoA carboxylase α with an alternative N-terminus in a tissue-restricted fashion [J].
Barber, MC ;
Travers, MT .
BIOCHEMICAL JOURNAL, 1998, 333 :17-25
[2]   Structure and regulation of acetyl-CoA carboxylase genes of metazoa [J].
Barber, MC ;
Price, NT ;
Travers, MT .
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS, 2005, 1733 (01) :1-28
[3]   The selective utilization of substrates in vivo by the phosphatidylethanolamine and phosphatidylcholine biosynthetic enzymes Ept1p and Cpt1p in yeast [J].
Boumann, HA ;
de Kruijff, B ;
Heck, AJR ;
de Kroon, AIPM .
FEBS LETTERS, 2004, 569 (1-3) :173-177
[4]   Transcriptome Analysis Reveals the Effect of Long Intergenic Noncoding RNAs on Pig Muscle Growth and Fat Deposition [J].
Chen, Guoting ;
Cheng, Xiaofang ;
Shi, Gaoli ;
Zola, Cheng ;
Chen, Lin ;
Li, Jingxuan ;
Li, Mengxun ;
Fang, Chengchi ;
Li, Changchun .
BIOMED RESEARCH INTERNATIONAL, 2019, 2019
[5]   Coordination of olfactory receptor choice with guidance receptor expression and function in olfactory sensory neurons [J].
Dang, Puneet ;
Fisher, Stephen A. ;
Stefanik, Derek ;
Kim, Junhyong ;
Raper, Jonathan A. .
PLOS GENETICS, 2018, 14 (01)
[6]   MMAPPR: Mutation Mapping Analysis Pipeline for Pooled RNA-seq [J].
Hill, Jonathon T. ;
Demarest, Bradley L. ;
Bisgrove, Brent W. ;
Gorsi, Bushra ;
Su, Yi-Chu ;
Yost, H. Joseph .
GENOME RESEARCH, 2013, 23 (04) :687-697
[7]   Transcriptome analysis reveals the long intergenic noncoding RNAs contributed to skeletal muscle differences between Yorkshire and Tibetan pig [J].
Huang, Ziying ;
Li, Qianqian ;
Li, Mengxun ;
Li, Changchun .
SCIENTIFIC REPORTS, 2021, 11 (01)
[8]   Novel Genomic Regions Associated with Intramuscular Fatty Acid Composition in Rabbits [J].
Laghouaouta, Houda ;
Sosa-Madrid, Bolivar Samuel ;
Zubiri-Gaitan, Agostina ;
Hernandez, Pilar ;
Blasco, Agustin .
ANIMALS, 2020, 10 (11) :1-17
[9]   Expression of placenta-specific 8 in human oocytes, embryos, and models of in vitro implantation [J].
Li, Min ;
Liu, Dong ;
Wang, Li ;
Wang, Weizhou ;
Wang, Aiming ;
Yao, Yuanqing .
FERTILITY AND STERILITY, 2016, 106 (03) :781-+
[10]   Stearoyl-CoA Desaturase Is Essential for Porcine Adipocyte Differentiation [J].
Liu, Lulu ;
Wang, Yu ;
Liang, Xiaojuan ;
Wu, Xiao ;
Liu, Jiali ;
Yang, Shulin ;
Tao, Cong ;
Zhang, Jin ;
Tian, Jianhui ;
Zhao, Jianguo ;
Wang, Yanfang .
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2020, 21 (07)
1234