Conjugation and radiolabeling of monoclonal antibodies with zirconium-89 for PET imaging using the bifunctional chelate p-isothiocyanatobenzyl-desferrioxamine

被引：377

作者：

Vosjan, Maria J. W. D. ^{[1
]}

Perk, Lars R. ^{[1
]}

Visser, Gerard W. M. ^{[2
,3
]}

Budde, Marianne ^{[1
]}

Jurek, Paul ^{[4
]}

Kiefer, Garry E. ^{[4
]}

van Dongen, Guus A. M. S. ^{[1
,2
,3
]}

机构：

[1] Vrije Univ Amsterdam, Med Ctr, Dept Otolaryngol Head & Neck Surg, Amsterdam, Netherlands

[2] Vrije Univ Amsterdam, Med Ctr, Dept Nucl Med, Amsterdam, Netherlands

[3] Vrije Univ Amsterdam, Med Ctr, PET Res, Amsterdam, Netherlands

[4] Macrocyclics, Dallas, TX USA

来源：

NATURE PROTOCOLS | 2010年 / 5卷 / 04期

基金：

美国国家科学基金会;

关键词：

POSITRON EMISSION TOMOGRAPHY; ZR-89; IMMUNO-PET;

D O I：

10.1038/nprot.2010.13

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The positron emitter zirconium-89 (Zr-89) has very attractive properties for positron emission tomography (PET) imaging of intact monoclonal antibodies (mAbs) using immuno-PET. This protocol describes the step-by-step procedure for the facile radiolabeling of mAbs or other proteins with Zr-89 using p-isothiocyanatobenzyl-desferrioxamine (Df-Bz-NCS). First, Df-Bz-NCS is coupled to the lysine-NH2 groups of a mAb at pH 9.0 (pre-modification), followed by purification using gel filtration. Next, the pre-modified mAb is labeled at room temperature by the addition of [Zr-89] Zr-oxalic acid solution followed by purification using gel filtration. The entire process of pre-modification, radiolabeling and purification steps will take about 2.5 h.

引用

页码：739 / 743

页数：5

共 19 条

[1] Disparity Between In Vivo EGFR Expression and 89Zr-Labeled Cetuximab Uptake Assessed with PET [J].

Aerts, Hugo J. W. L. ;

Dubois, Ludwig ;

Perk, Lars ;

Vermaelen, Peter ;

van Dongen, Guus A. M. S. ;

Wouters, Bradly G. ;

Lambin, Philippe .

JOURNAL OF NUCLEAR MEDICINE, 2009, 50 (01) :123-131

[2] Radiation Dosimetry of 89Zr-Labeled Chimeric Monoclonal Antibody U36 as Used for Immuno-PET in Head and Neck Cancer Patients [J].

Boerjesson, Pontus K. E. ;

Jauw, Yvonne W. S. ;

de Bree, Remco ;

Roos, Jan C. ;

Castelijns, Jonas A. ;

Leemans, C. Rene ;

van Dongen, Guus A. M. S. ;

Boellaard, Ronald .

JOURNAL OF NUCLEAR MEDICINE, 2009, 50 (11) :1828-1836

[3] Performance of immuno-positron emission tomography with zirconium-89-labeled chimeric monoclonal antibody U36 in the detection of lymph node metastases in head and neck cancer patients [J].

Börjesson, PKE ;

Jauw, YWS ;

Boellaard, R ;

de Bree, R ;

Comans, EFI ;

Roos, JC ;

Castelijns, JA ;

Vosjan, MJWD ;

Kummer, JA ;

Leemans, CR ;

Lammertsma, AA ;

van Dongen, GAMS .

CLINICAL CANCER RESEARCH, 2006, 12 (07) :2133-2140

[4] PET radioimmunoscintigraphy of renal cell cancer using 89Zr-labeled cG250 monoclonal antibody in nude rats [J].

Brouwers, A ;

Verel, I ;

Van Eerd, J ;

Visser, G ;

Steffens, M ;

Oosterwijk, E ;

Corstens, F ;

Oyen, W ;

Van Dongen, G ;

Boerman, O .

CANCER BIOTHERAPY AND RADIOPHARMACEUTICALS, 2004, 19 (02) :155-163

[5] Potent antibody therapeutics by design [J].

Carter, PJ .

NATURE REVIEWS IMMUNOLOGY, 2006, 6 (05) :343-357

[6] Development and Characterization of Clinical-Grade 89Zr-Trastuzumab for HER2/neu ImmunoPET Imaging [J].

Dijkers, Eli C. F. ;

Kosterink, Jos G. W. ;

Rademaker, Anna P. ;

Perk, Lars R. ;

van Dongen, Guus A. M. S. ;

Bart, Joost ;

de Jong, Johan R. ;

de Vries, Elisabeth G. E. ;

Lub-de Hooge, Marjolijn N. .

JOURNAL OF NUCLEAR MEDICINE, 2009, 50 (06) :974-981

[7] In vivo VEGF imaging with radiolabeled bevacizumab in a human ovarian tumor xenograft [J].

Nagengast, Wouter B. ;

de Vries, Elisabeth G. ;

Hospers, Geke A. ;

Mulder, Nanno H. ;

de Jong, Johan R. ;

Hollema, Harry ;

Brouwers, Adrienne H. ;

van Dongen, Guns A. ;

Perk, Lars R. ;

Lub-de Hooge, Marjolijn N. .

JOURNAL OF NUCLEAR MEDICINE, 2007, 48 (08) :1313-1319

[8] Radioimmunoimaging with Longer-Lived Positron-Emitting Radionuclides: Potentials and Challenges [J].

Nayak, Tapan K. ;

Brechbiel, Martin W. .

BIOCONJUGATE CHEMISTRY, 2009, 20 (05) :825-841

[9] Preparation and evaluation of 89Zr-Zevalin for monitoring of 90Y-Zevalin biodistribution with positron emission tomography [J].

Perk, Lars R. ;

Visser, Otto J. ;

Walsum, M. Stigter-van ;

Vosjan, Maria J. W. D. ;

Visser, Gerard W. M. ;

Zijlstra, Josee M. ;

Huijgens, Peter C. ;

van Dongen, Guus A. M. S. .

EUROPEAN JOURNAL OF NUCLEAR MEDICINE AND MOLECULAR IMAGING, 2006, 33 (11) :1337-1345

[10] p-Isothiocyanatobenzyl-desferrioxamine: a new bifunctional chelate for facile radiolabeling of monoclonal antibodies with zirconium-89 for immuno-PET imaging [J].

Perk, Lars R. ;

Vosjan, Maria J. W. D. ;

Visser, Gerard W. M. ;

Budde, Marianne ;

Jurek, Paul ;

Kiefer, Garry E. ;

van Dongen, Guus A. M. S. .

EUROPEAN JOURNAL OF NUCLEAR MEDICINE AND MOLECULAR IMAGING, 2010, 37 (02) :250-259

← 1 2 →