Annular aperture-detected coherent anti-Stokes Raman scattering microscopy for high contrast vibrational imaging

被引：7

作者：

Lin, Jian ^{[1
]}

Lu, Fake ^{[1
]}

Zheng, Wei ^{[1
]}

Huang, Zhiwei ^{[1
]}

机构：

[1] Natl Univ Singapore, Dept Bioengn, Opt Bioimaging Lab, Fac Engn, Singapore 117576, Singapore

来源：

APPLIED PHYSICS LETTERS | 2010年 / 97卷 / 08期

基金：

英国医学研究理事会;

关键词：

biological techniques; cellular biophysics; coherent antiStokes Raman scattering; optical microscopy; Raman spectroscopy; POLARIZATION;

D O I：

10.1063/1.3483772

中图分类号：

O59 [应用物理学];

学科分类号：

摘要：

We report on a unique annular aperture detection scheme for effective suppression of nonresonant background in coherent anti-Stokes Raman scattering (CARS) microscopy imaging. The results show that the resonant CARS signal to nonresonant background ratio (SBR) varies with both the scatterers' sizes and the annular aperture diameters used, and an approximately 20-fold improvement in SBR can be obtained when an annular aperture size of 0.9 (related to the objective diameter) is selected for imaging the scatterers with diameters close to the excitation wavelength. We demonstrate this technique by imaging 300 to 1100 nm polystyrene beads and human epithelial cells in aqueous environments. (C) 2010 American Institute of Physics. [doi:10.1063/1.3483772]

引用

页数：3

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