Imaging voltage-dependent cell motions with heterodyne Mach-Zehnder phase microscopy

被引：48
作者：
Fang-Yen, Christopher
Oh, Seungeun
Park, Yongkeun
Choi, Wonshik
Song, Sen
Seung, H. Sebastian
Dasari, Ramachandra R.
Feld, Michael S.
机构：
[1] MIT, GR Harrison Spect Lab, Cambridge, MA 02139 USA
[2] MIT, Howard Hughes Med Inst, Cambridge, MA 02139 USA
[3] MIT, Dept Brain & Cognit Sci, Cambridge, MA 02139 USA
来源：
OPTICS LETTERS | 2007年 / 32卷 / 11期
关键词：
D O I：
10.1364/OL.32.001572
中图分类号：
O43 [光学];
学科分类号：
070207 ; 0803 ;
摘要：
We describe a heterodyne Mach-Zehnder interferometric microscope capable of quantitative phase imaging of biological samples with subnanometer sensitivity and frame rates up to 10 kHz. We use the microscope to image cultured neurons and measure nanometer-scale voltage-dependent motions in cells expressing the membrane motor protein prestin. (C) 2007 Optical Society of America
引用
收藏
页码：1572 / 1574
页数：3
相关论文
共 22 条
[1]   Detection of neural activity using phase-sensitive optical low-coherence reflectometry [J].
Akkin, T ;
Davé, DP ;
Milner, TE ;
Rylander, HG .
OPTICS EXPRESS, 2004, 12 (11) :2377-2386
[2]   Quantitative phase-amplitude microscopy I: optical microscopy [J].
Barone-Nugent, ED ;
Barty, A ;
Nugent, KA .
JOURNAL OF MICROSCOPY-OXFORD, 2002, 206 :194-203
[3]   Quantitative optical phase microscopy [J].
Barty, A ;
Nugent, KA ;
Paganin, D ;
Roberts, A .
OPTICS LETTERS, 1998, 23 (11) :817-819
[4]   Spectral-domain phase microscopy [J].
Choma, MA ;
Ellerbee, AK ;
Yang, CH ;
Creazzo, TL ;
Izatt, JA .
OPTICS LETTERS, 2005, 30 (10) :1162-1164
[5]   Prestin, a new type of motor protein [J].
Dallos, P ;
Fakler, B .
NATURE REVIEWS MOLECULAR CELL BIOLOGY, 2002, 3 (02) :104-111
[6]  
DUNN GA, 1995, J CELL SCI, V108, P1239
[7]   Noncontact measurement of nerve displacement during action potential with a dual-beam low-coherence interferometer [J].
Fang-Yen, C ;
Chu, MC ;
Seung, HS ;
Dasari, RR ;
Feld, MS .
OPTICS LETTERS, 2004, 29 (17) :2028-2030
[8]   SATELLITE RADAR INTERFEROMETRY - TWO-DIMENSIONAL PHASE UNWRAPPING [J].
GOLDSTEIN, RM ;
ZEBKER, HA ;
WERNER, CL .
RADIO SCIENCE, 1988, 23 (04) :713-720
[9]   Hilbert phase microscopy for investigating fast dynamics in transparent systems [J].
Ikeda, T ;
Popescu, G ;
Dasari, RR ;
Feld, MS .
OPTICS LETTERS, 2005, 30 (10) :1165-1167
[10]   Quantitative phase imaging using actively stabilized phase-shifting low-coherence interferometry [J].
Iwai, H ;
Fang-Yen, C ;
Popescu, G ;
Wax, A ;
Badizadegan, K ;
Dasari, RR ;
Feld, MS .
OPTICS LETTERS, 2004, 29 (20) :2399-2401
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