Evaluation of the effect of particle size, aeration rate and harvest time on the production of cellulase by Trichoderma reesei QM9414 using response surface methodology

被引：24

作者：

Rocky-Salimi, K. ^{[1
]}

Hamidi-Esfahani, Z. ^{[1
]}

机构：

[1] Tarbiat Modares Univ, Fac Agr, Dept Food Sci & Technol, Tehran, Iran

来源：

FOOD AND BIOPRODUCTS PROCESSING | 2010年 / 88卷 / C1期

关键词：

Aeration rate; Harvesting time; Particle size; Response surface methodology; Rice bran; Trichoderma reesei QM9414; SOLID-STATE FERMENTATION; LIGNOCELLULOSIC BIOMASS; PENICILLIUM; CULTURE; MUTANTS;

D O I：

10.1016/j.fbp.2009.06.006

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

In the present study, the effect of culture conditions on cellulase activities by Trichoderma reesei QM9414 grown on rice bran media in solid-state fermentation was evaluated. Also, the optimum conditions were determined using response surface methodology (RSM). A face center design was used to investigate the effect of independent variables i.e. particle size, aeration rate and harvesting time on filter paper activity (EPA), Avicelase and carboxy methyl cellulase (CMCase) activities. The highest FPA, Avicelase and CMCase activities were 11.648, 99.756 and 94.210 U/g dry weight of substrate (DWS), respectively. The results showed that aeration rate and particle size had significant effects on the activity of cellulase on FPA. Also, particle size and harvesting time had significant effects on the activity of both Avicelase and CMCase. The reduction of particle size and harvesting time to 1-1.5 mm and 4 days, respectively increased cellulase activities. (C) 2009 The Institution of Chemical Engineers. Published by Elsevier B.V. All rights reserved.

引用

页码：61 / 66

页数：6

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