Direct quantitation of poly(ADP-Ribose) polymerase (PARP) activity as a means to distinguish necrotic and apoptotic death in cell and tissue samples

被引：32

作者：

Putt, KS

Beilman, GJ

Hergenrother, PJ ^{[1
]}

机构：

[1] Univ Illinois, Dept Biochem, Urbana, IL 61801 USA

[2] Univ Minnesota, Dept Surg, Minneapolis, MN 55455 USA

[3] Univ Illinois, Dept Chem, Urbana, IL 61801 USA

来源：

CHEMBIOCHEM | 2005年 / 6卷 / 01期

关键词：

apoptosis; bioorganic chemistry; enzymes; necrosis; PARP-1;

D O I：

10.1002/cbic.200400330

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

PARP-angel of death. The PARP-1 enzyme is either inactivated or activated depending on whether a cell dies via apoptosis or necrosis (see scheme). This differential processing of PARP-1 can now be used as a rapid, sensitive, and convenient method to distinguish apoptotic from necrotic cell death, and is even applied to quantitate necrosis in bulk tissue from a pig in which a hemorrhagic shock was induced.

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收藏

页码：53 / 55

页数：3

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