Immune responses in pigs induced by recombinant canine adenovirus 2 expressing the glycoprotein 5 of porcine reproductive and respiratory syndrome virus

被引：15

作者：

Zhou, J. -X. ^{[1
,4
]}

Xue, J. -D. ^{[1
,2
]}

Yu, T. ^{[5
]}

Zhang, J. -B. ^{[3
]}

Liu, Y. ^{[1
]}

Jiang, N. ^{[1
]}

Li, Y. -L. ^{[5
]}

Hu, R. -L. ^{[1
]}

机构：

[1] Acad Mil Med Sci, Vet Res Inst, Lab Epidemiol, Changchun 130062, Jilin, Peoples R China

[2] Inner Mongolia Univ Nationalities, Coll Anim Sci & Technol, Tongliao, Peoples R China

[3] Jilin Univ, Ctr Expt Anim, Changchun, Peoples R China

[4] JiLin Agr Univ, Coll Anim Sci & Technol, Changchun, Peoples R China

[5] JiLin Agr Univ, Coll Life Sci, Changchun, Peoples R China

来源：

VETERINARY RESEARCH COMMUNICATIONS | 2010年 / 34卷 / 04期

基金：

中国国家自然科学基金;

关键词：

Porcine reproductive and respiratory syndrome virus; Glycoprotein; 5; Canine adenovirus vector; Pigs; Recombinant vaccine; Immunization; NORTH-AMERICAN; PRRS VIRUS; PROTEINS; GP5; VACCINATION; ANTIBODY; EFFICACY; ORF5;

D O I：

10.1007/s11259-010-9364-7

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

To develop a new type vaccine for porcine reproductive and respiratory syndrome (PRRS) prevention by using canine adenovirus 2(CAV-2) as vector, the Glycoprotein 5(GP5) gene from PRRSV strain JL was amplified by RT-PCR, and the expression cassette of GP5 was constructed using the human cytomegalovirus (HCMV) promoter and the simian virus 40 (SV40) early mRNA polyadenylation signal. The expression cassette of Glycoprotein 5 was cloned into the CAV-2 genome in which E3 region had been partly deleted, and the recombinant virus (CAV-2-GP5) was obtained by transfecting the recombinant CAV-2-GP5 genome into MDCK cells together with Lipofectamine(TM) 2000. Immunization trial in pigs with the recombinant virus CAV-2-GP5 showed that CAV-2-GP5 could stimulate a specific immune response to PRRSV. Immune response to the GP5 and PRRSV was confirmed by ELISA, neutralization test and lymphocyte proliferative responses, and western blotting confirmed expression of GP5 by the vector in cells. These results indicated that CAV-2 may serve as a vector for development of PRRSV vaccine in pigs, and the CAV-2-GP5 might be a candidate vaccine to be tested for preventing PRRSV infection.

引用

页码：371 / 380

页数：10

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