Enhanced enantioselectivity of Candida rugosa lipase in ionic liquids as compared to organic solvents

被引：72

作者：

Ulbert, O ^{[1
]}

Fráter, T ^{[1
]}

Bélafi-Bakó, K ^{[1
]}

Gubicza, L ^{[1
]}

机构：

[1] Univ Veszprem, Chem Proc Engn Res Inst, H-8201 Veszprem, Hungary

来源：

JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC | 2004年 / 31卷 / 1-3期

基金：

匈牙利科学研究基金会;

关键词：

ionic liquid; Candida rugosa lipase; enantioselective esterification; water activity; hydrophobicity;

D O I：

10.1016/j.molcatb.2004.07.003

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Candida rugosa lipase has shown to retain catalytic activity in ionic liquids. In this work, enantioselectivity of this enzyme in the esterific action of 2-substituted-propanoic acids and 1-butanol is compared in ionic liquids and organic solvents. The role of solvent hydrophobicity (log P), water content and the effect of substituents are evaluated. Optimal water concentration in the reaction media was determined, where the enzyme shows maximal activity and enantioselectivity. Enantioselectivity can be improved when chlorine substituent was replaced by slightly bigger size bromine. Contrary to reactions in common organic solvents, there was no need for purification steps following the reaction in ionic liquids in order to recycle the enzyme. In 1-butyl-3-methyl-inidazolium-hexafluoro-phosphate ([bmim]PF6) and 1-octyl-3-nDnylimidazohum-hexafiuoro-phosphate ([onim]PF6) ionic liquids, C. rugosa lipase could be recycled five times without appreciable activity or enantioselectivity losses. (C) 2004 Elsevier B.V All rights reserved.

引用

页码：39 / 45

页数：7

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