Mechanisms and phenotypic consequences of acquisition of tigecycline resistance by Stenotrophomonas maltophilia

被引:18
|
作者
Blanco, Paula [1 ]
Corona, Fernando [1 ]
Luis Martinez, Jose [1 ]
机构
[1] CSIC, Ctr Nacl Biotecnol, Madrid 28049, Spain
关键词
MULTIDRUG EFFLUX PUMP; RIBOSOMAL S10 PROTEIN; CYSTIC-FIBROSIS; ANTIBIOTIC-RESISTANCE; SEQUENCE-ANALYSIS; BETA-LACTAMASE; FITNESS COSTS; SMEDEF; EMERGENCE; INFECTIONS;
D O I
10.1093/jac/dkz326
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
Objectives: To elucidate the potential mutation-driven mechanisms involved in the acquisition of tigecycline resistance by the opportunistic pathogen Stenotrophomonas maltophilia. The mutational trajectories and their effects on bacterial fitness, as well as cross-resistance and/or collateral susceptibility to other antibiotics, were also addressed. Methods: S. maltophilia populations were submitted to experimental evolution in the presence of increasing concentrations of tigecycline for 30days. The genetic mechanisms involved in the acquisition of tigecycline resistance were determined by WGS. Resistance was evaluated by performing MIC assays. Fitness of the evolved populations and individual clones was assessed by measurement of the maximum growth rates. Results: All the tigecycline-evolved populations attained high-level resistance to tigecycline following different mutational trajectories, yet with some common elements. Among the mechanisms involved in low susceptibility to tigecycline, mutations in the SmeDEF efflux pump negative regulator smeT, changes in proteins involved in the biogenesis of the ribosome and modifications in the LPS biosynthesis pathway seem to play a major role. Besides tigecycline resistance, the evolved populations presented cross-resistance to other antibiotics, such as aztreonam and quinolones, and they were hypersusceptible to fosfomycin, suggesting a possible combination treatment. Further, we found that the selected resistance mechanisms impose a relevant fitness cost when bacteria grow in the absence of antibiotic. Conclusions: Mutational resistance to tigecycline was easily selected during exposure to this antibiotic. However, the fitness cost may compromise the maintenance of S. maltophilia tigecycline-resistant populations in the absence of antibiotic.
引用
收藏
页码:3221 / 3230
页数:10
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