Cloning and characterization of the lactate dehydrogenase genes from Lactobacillus sp RKY2

被引：9

作者：

Lee, JH

Choi, MH

Kang, HK

Ryu, HW

Sunwo, CS

Wee, YJ

Park, KD

Kim, DW

Kim, D ^{[1
]}

机构：

[1] Chonnam Natl Univ, Inst Engn Res, Kwangju 500757, South Korea

[2] Chonnam Natl Univ, Dept Mat & Biochem Engn, Kwangju 500757, South Korea

[3] Chonnam Natl Univ, Sch Biol Sci & Technol, Kwangju 500757, South Korea

[4] Chonnam Natl Univ, Res Inst Catalysis, Kwangju 500757, South Korea

[5] Chonnam Natl Univ, Korea Basic Sci Inst Gwang Ju Branch, Kwangju 500757, South Korea

[6] Kangnung Natl Univ, Dept Phys, Kangnung 210702, South Korea

[7] Dongshin Univ, Biol Res Ctr Ind Accelerator, Naju 520714, Jeonnam, South Korea

来源：

BIOTECHNOLOGY AND BIOPROCESS ENGINEERING | 2004年 / 9卷 / 04期

关键词：

lactic acid; lactate dehydrogenase; Lactobacillus; homofermentative; gene; cloning;

D O I：

10.1007/BF02942351

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Lactic acid is an environmentally benign organic acid that could be used as a raw material for biodegradable plastics if it can be inexpensively produced by fermentation. Two genes (IdhL and IdhD) encoding the L-(+) and D-(-) lactate dehydrogenases (L-LDH and D-LDH) were cloned from Lactobacillus sp., RKY2, which is a lactic acid hyper-producing bacterium isolated from Kimchi. Open reading frames of IdhL for and IdhD for the L and D-LDH genes were 962 and 998 bp, respectively. Both the L(+)- and D(-)-LDH proteins showed the highest degree of homology with the L- and D-lactate dehydrogenase genes of Lactobacillus plantarum. The conserved residues in the catalytic activity and substrate binding of both LDHs were identified in both enzymes.

引用

页码：318 / 322

页数：5

共 20 条

[1] CLONING OF THE D-LACTATE DEHYDROGENASE GENE FROM LACTOBACILLUS-DELBRUECKII SUBSP BULGARICUS BY COMPLEMENTATION IN ESCHERICHIA-COLI [J].