An asymmetric electrospun membrane for the controlled release of ciprofloxacin and FGF-2: Evaluation of antimicrobial and chemoattractant properties

被引：23

作者：

Federico, Salvatore ^{[1
]}

Pitarresi, Giovanna ^{[1
]}

Palumbo, Fabio S. ^{[1
]}

Fiorica, Calogero ^{[1
]}

Catania, Valentina ^{[1
]}

Schillaci, Domenico ^{[1
]}

Giammona, Gaetano ^{[1
,2
]}

机构：

[1] Univ Palermo, Dipartimento Sci & Tecnol Biol Chim & Farmaceut S, Via Archirafi 32, I-90123 Palermo, Italy

[2] Italian Natl Res Council, Inst Biophys Palermo, Via Ugo La Malfa 153, I-90146 Palermo, Italy

来源：

MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS | 2021年 / 123卷

关键词：

Gellan gum alkyl-derivative; Polyurethanes; Double layer electrospun membrane; Chemotaxis (FGF-2); Antimicrobial activity (CPX);

D O I：

10.1016/j.msec.2021.112001

中图分类号：

TB3 [工程材料学]; R318.08 [生物材料学];

学科分类号：

0805 ; 080501 ; 080502 ;

摘要：

Here, an asymmetric double-layer membrane has been designed and fabricated by electrospinning as a tool for a potential wound healing application. A hydrophobic layer has been produced by using a polyurethanepolycaprolactone (PU-PCL) copolymer and loaded with the antibacterial ciprofloxacin whereas an ion responsive hydrophilic layer has been produced by using an octyl derivative of gellan gum (GG-C8) and polyvinyl alcohol (PVA) and loaded with the growth factor FGF-2. This study investigated how the properties of this asymmetric membrane loaded with actives, were influenced by the ionotropic crosslinking of the hydrophilic layer. In particular, the treatment in DPBS and the crosslinking in CaCl2 0.1 or 1 M of the hydrophilic layer affected the release profile of the bioactive molecules allowing to modulate both the antimicrobial effect, as assayed by logarithmic reduction of the Staphylococcus aureus viable count, and the chemoattractant properties on NIH 3 T3 cell line, as assayed by scratch test and cell chemoattraction assay.

引用

页数：11

共 58 条

[1] Synthesis and evaluation of thermo-rheological behaviour and ionotropic crosslinking of new gellan gum-alkyl derivatives [J].