Treadmill pre-training suppresses the release of glutamate resulting from cerebral ischemia in rats

被引：26

作者：

Jia, Jie ^{[1
,2
]}

Zhang, Feng ^{[1
]}

Hu, Yong-Shan ^{[1
,2
]}

Wu, Yi ^{[2
]}

Yu, Hui-Xian ^{[1
]}

Liu, Gang ^{[1
]}

Zhu, Da-Nian ^{[3
]}

Xia, Chun-Mei ^{[3
]}

Cao, Zhi-Juan ^{[4
]}

Zhang, Xi ^{[5
]}

Guo, Qing-Chuan ^{[5
]}

机构：

[1] Fudan Univ, Huashan Hosp, Dept Rehabil, Shanghai 200433, Peoples R China

[2] Fudan Univ, State Key Lab Med Neurobiol, Shanghai, Peoples R China

[3] Fudan Univ, Coll Med, Dept Physiol & Pathophysiol, Shanghai 200433, Peoples R China

[4] Fudan Univ, Coll Pharm, Shanghai 200433, Peoples R China

[5] Third Hosp, Xinxiang Med Coll, Dept Rehabil, Xinxiang, Henan, Peoples R China

来源：

EXPERIMENTAL BRAIN RESEARCH | 2010年 / 204卷 / 02期

基金：

国家高技术研究发展计划(863计划); 中国国家自然科学基金;

关键词：

Brain infarction; Ischemia/reperfusion; Metabotropic glutamate receptor; Neuroprotection; OXYGEN-GLUCOSE DEPRIVATION; RECEPTOR ANTAGONIST; ARTERY OCCLUSION; TRANSPORTER; EXPRESSION; BRAIN; TOLERANCE; CHANNELS; INJURY;

D O I：

10.1007/s00221-010-2320-5

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

This study was designed to investigate the neuroprotective effect of treadmill pre-training against the over-release of glutamate resulting from cerebral ischemia. Sprague-Dawley rats underwent 2 weeks of treadmill run-training before cerebral ischemia was performed by middle cerebral artery occlusion. The level of glutamate in brain extracellular fluid was detected before, during and after ischemia/reperfusion. The expression of metabotropic glutamate receptor-1 (mGluR1) mRNA in striatum was examined after ischemia for 80 min and reperfusion for 240 min. Neurological defect score and brain infarction volumes were measured. The treadmill pre-training significantly suppressed the release of glutamate, and reduced the expression of mGluR1 mRNA at 59% (P < 0.01) and 62% (P < 0.05), respectively, as compared with the ischemia group. The neurological defect score and infarction volume were significantly improved by 75% (P < 0.01) and 74% (P < 0.01), respectively, in the pre-training group, as compared to the ischemia group. Treadmill pre-training has a significant neuroprotective function against ischemia/reperfusion injury, by suppressing glutamate release resulting from cerebral ischemia, and this effect may be mediated by downregulation of mGluR1.

引用

页码：173 / 179

页数：7

共 42 条

[1] Selective mGluR5 receptor antagonist or agonist provides neuroprotection in a rat model of focal cerebral ischemia [J].

Bao, WL ;

Williams, AJ ;

Faden, AI ;

Tortella, FC .

BRAIN RESEARCH, 2001, 922 (02) :173-179

[2] Group I metabotropic glutamate receptors reduce excitotoxic injury and may facilitate neurogenesis [J].

Baskys, A ;

Bayazitov, D ;

Fang, L ;

Blaabjerg, M ;

Poulsen, FR ;

Zimmer, J .

NEUROPHARMACOLOGY, 2005, 49 :146-156

[3] Investigation of the modulation of glutamate release by sodium channels using neurotoxins [J].

Bicalho, AFX ;

Guatimosim, C ;

Prado, MAM ;

Gomez, MV ;

Romano-Silva, MA .

NEUROSCIENCE, 2002, 113 (01) :115-123

[4] Ionotropic glutamate receptors and glutamate transporters are involved in necrotic neuronal cell death induced by oxygen-glucose deprivation of hippocampal slice cultures [J].

Bonde, C ;

Noraberg, J ;

Noer, H ;

Zimmer, J .

NEUROSCIENCE, 2005, 136 (03) :779-794

[5] mGluRI targets microglial activation and selectively prevents neuronal cell engulfment through Akt and caspase dependent pathways [J].

Chong, ZZ ;

Kang, JQ ;

Li, FQ ;

Maiese, K .

CURRENT NEUROVASCULAR RESEARCH, 2005, 2 (03) :197-211

[6] Histological studies of the effects of monosodium glutamate of the Fallopian tubes of adult female Wistar rats [J].

Eweka, Andrew Osayame ;

Eweka, Abieyuwa ;

Om'Iniabohs, Ferdinard A. E. .

NORTH AMERICAN JOURNAL OF MEDICAL SCIENCES, 2010, 2 (03) :146-149

[7] AN EXCITATORY AMINO-ACID TRANSPORTER WITH PROPERTIES OF A LIGAND-GATED CHLORIDE CHANNEL [J].

FAIRMAN, WA ;

VANDENBERG, RJ ;

ARRIZA, JL ;

KAVANAUGH, MP ;

AMARA, SG .

NATURE, 1995, 375 (6532) :599-603

[8] Mechanisms of oxygen glucose deprivation-induced glutamate release from cerebrocortical slice cultures [J].

Fujimoto, S ;

Katsuki, H ;

Kume, T ;

Kaneko, S ;

Akaike, A .

NEUROSCIENCE RESEARCH, 2004, 50 (02) :179-187

[9] Real-time measurement of glutamate release from the ischemic penumbra of the rat cerebral cortex using a focal middle cerebral artery occlusion model [J].

Guyot, LL ;

Diaz, FG ;

O'Regan, MH ;

McLeod, S ;

Park, H ;

Phillis, JW .

NEUROSCIENCE LETTERS, 2001, 299 (1-2) :37-40

[10] Introduction: Glutamate transport, metabolism, and physiological responses [J].

Hediger, MA ;

Welbourne, TC .

AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY, 1999, 277 (04) :F477-F479

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