Characterization and epitope mapping of monoclonal antibodies raised against rat hepatitis E virus capsid protein: An evaluation of their neutralizing activity in a cell culture system

被引：16

作者：

Kobayashi, Tominari ^{[1
]}

Takahashi, Masaharu ^{[1
]}

Tanggis ^{[1
]}

Mulyanto ^{[2
,3
]}

Jirintai, Suljid ^{[1
]}

Nagashima, Shigeo ^{[1
]}

Nishizawa, Tsutomu ^{[1
]}

Okamoto, Hiroaki ^{[1
]}

机构：

[1] Jichi Med Univ, Div Virol, Dept Infect & Immun, Sch Med, 3311-1 Yakushiji, Shimotsuke, Tochigi 3290498, Japan

[2] West Nusa Tenggara Hepatitis Lab, Mataram, Indonesia

[3] Univ Mataram, Immunol Lab, Fac Med, Mataram, Indonesia

来源：

JOURNAL OF VIROLOGICAL METHODS | 2016年 / 233卷

关键词：

Rat hepatitis E virus; Monoclonal antibodies; Capsid protein; Epitope mapping; Neutralization; WILD RATS; IDENTIFICATION; CLASSIFICATION; GLYCOSYLATION; EXPRESSION; PARTICLES; INFECTION; STRAINS; FAMILY; SITES;

D O I：

10.1016/j.jviromet.2016.03.004

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Hepatitis E virus (HEV) is the causative agent of acute hepatitis. Rat HEV is a recently discovered virus related to, but distinct from, human HEV. Since laboratory rats can be reproducibly infected with rat HEV and a cell culture system has been established for rat HEV, this virus may be used as a surrogate virus for human HEV, enabling studies on virus replication and mechanism of infection. However, monoclonal antibodies (MAbs) against rat HEV capsid (ORF2) protein are not available. In this study, 12 murine MAbs were generated against a recombinant ORF2 protein of rat HEV (rRatHEV-ORF2: amino acids 101-644) and were classified into at least six distinct groups by epitope mapping and a cross-reactivity analysis with human HEV ORF2 proteins. Two non-cross-reactive MAbs recognizing the protruding (P) domain detected both non-denatured and denatured rRatHEV-ORF2 protein and efficiently captured cell culture produced rat HEV particles that had been treated with deoxycholate and trypsin, but not those without prior treatment. In addition, these two MAbs were able to efficiently neutralize replication of cell culture generated rat HEV particles without lipid membranes (but not those with lipid membranes) in a cell culture system, similar to human HEV. (C) 2016 Elsevier B.V. All rights reserved.

引用

页码：78 / 88

页数：11

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