Molecular characterization of the sugarcane cultivars obtained by issr markers

被引：11

作者：

Alves de Almeida, Clebia Maria ^{[1
]}

Nascimento de Lima, Sue Ellen ^{[2
]}

de Andrade Lima, Gaus Silvestre ^{[3
]}

de Brito, Julio Zoe ^{[4
]}

Tenorio Sabino Donato, Virginia Maria ^{[5
]}

da Silva, Marcia Vanusa ^{[6
]}

机构：

[1] Univ Fed Pernambuco UFPE, CCB, PPGCB, BR-50670901 Recife, PE, Brazil

[2] IPA, Lab Biol Solo, Dept Biotecnol, BR-50761000 Recife, PE, Brazil

[3] Univ Fed Alagoas, Ctr Ciencias Agr, Dept Fitossanidade, BR-57100000 Rio Largo, AI, Brazil

[4] IPA, Lab Cultura Tecidos, BR-50761000 Recife, PE, Brazil

[5] Univ Fed Rural Pernambuco, Dept Biol, BR-52171900 Recife, PE, Brazil

[6] Univ Fed Pernambuco, Dept Bioquim, CCB, BR-50650420 Recife, PE, Brazil

来源：

CIENCIA E AGROTECNOLOGIA | 2009年 / 33卷

关键词：

Saccharum oficinarum; molecular markers; genetic diversity; GENETIC DIVERSITY; RAPD MARKERS; AMPLIFICATION; IDENTIFICATION; POLYMORPHISM; ORYZA;

D O I：

10.1590/S1413-70542009000700012

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

Genetic diversity of fourteen sugarcane cultivars was accessed by ISSR molecular markers. With the aim to characterizing and validating the efficiency of these markers in the fingerprint of studied cultivars, thirty seven ISSR primers were used, from which, eight were efficient for the DNA amplification. Seven primers were efficient to discriminate the fourteen studied sugarcane cultivars. The amplicons varied from 300 to 2000 bp. The cultivars RB 92579 and RB 863129 presented higher similarity coefficient (77%) while the cultivars RB 961 and RB 93611 formed the group with lower similarity (22%). The results suggested that ISSR markers were useful in the analysis of the genetic diversity and in the fingerprint in sugarcane germosplasm. In the next step more ISSR primers will be used in order to obtain more polymorphism from these varieties and to analyze more sugarcane cultivars.

引用

页码：1771 / 1776

页数：6

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